| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,778,392 | T→A | 100% | R145R (CGA→CGT) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,778,392 | 0 | T | A | 87.0% | 142.8 / 12.7 | 69 | R145R (CGA→CGT) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base A (33/27); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAAAGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAC > minE/2778330‑2778457 | aaaacGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATc < 1:3072135/69‑1 (MQ=255) aaGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCa < 1:936388/67‑1 (MQ=255) aaGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCa < 1:839964/67‑1 (MQ=255) aaGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCa < 1:3431130/67‑1 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGt < 1:3266981/69‑1 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACt > 1:496512/1‑67 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTg > 1:1500035/1‑68 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTg > 1:734567/1‑68 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:53962/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2599589/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:543750/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:76228/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:292829/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2612643/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2433540/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2236871/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2230447/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:1159596/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:1502798/1‑69 (MQ=255) gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:182025/1‑69 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:555548/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:3395805/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:15738/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:451871/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:47676/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2660445/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1664838/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2612625/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:259482/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1309327/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2402742/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:6095/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2376336/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:804213/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2001757/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1967608/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGat > 1:195780/1‑67 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTAAGTACACTGTACCTGGTCGata > 1:2654018/1‑68 (MQ=255) ggCAATTTACCGCCGATAGGCGCGGACATTCGCATc < 1:2337729/36‑1 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCTTGAtt < 1:1191529/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1086197/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1499242/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:663116/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:474219/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1885457/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:380870/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:3214842/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2086414/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2176025/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:170551/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2613155/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2396155/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1581740/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2414330/69‑2 (MQ=255) ccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2712785/68‑2 (MQ=255) aGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:578631/63‑2 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1697486/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:49699/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:658393/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1200686/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:750490/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1592801/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:2000150/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:2752115/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:1590111/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGGCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:678819/69‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1040615/68‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:2220756/68‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:8643/68‑1 (MQ=255) | AAAAAGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAC > minE/2778330‑2778457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |