| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,778,401 | A→G | 58.9% | H142H (CAT→CAC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,778,401 | 0 | A | G | 58.9% | 42.3 / 72.5 | 102 | H142H (CAT→CAC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (12/30); new base G (33/27); total (45/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTG > minE/2778345‑2778467 | gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGt < 1:3266981/69‑1 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACt > 1:496512/1‑67 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTg > 1:734567/1‑68 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTg > 1:1500035/1‑68 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:53962/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:182025/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:292829/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2612643/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:1502798/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:543750/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2599589/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2433540/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2230447/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:1159596/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:76228/1‑69 (MQ=255)gCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGt > 1:2236871/1‑69 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:3395805/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2402742/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2612625/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2376336/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2001757/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1967608/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:2660445/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:47676/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:804213/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:6095/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1309327/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:555548/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:15738/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:1664838/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGata > 1:451871/1‑68 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGat > 1:195780/1‑67 (MQ=255) gCATCGGCAATTTACCGCCGATAGGCGCGGACATACGCATCAGGTAAGTACACTGTACCTGGTCGata > 1:2654018/1‑68 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCTTGAtt < 1:1191529/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2613155/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:474219/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1086197/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2414330/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:380870/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2396155/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:663116/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:3214842/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:170551/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2176025/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1581740/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1499242/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:1885457/69‑2 (MQ=255) gccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2086414/69‑2 (MQ=255) ccgATAGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:2712785/68‑2 (MQ=255) aGGCGCGGACATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGAtt < 1:578631/63‑2 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1200686/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:49699/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:2000150/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:658393/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:750490/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1592801/69‑1 (MQ=255) aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1697486/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:2752115/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:1590111/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGGCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:678819/69‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:2220756/68‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1040615/68‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:8643/68‑1 (MQ=255) cGCATCAGATGCTTACACTGTACCTGGTCGATAATAATCg < 1:2933846/40‑1 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:3399816/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:2342562/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:1343101/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:1930900/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:3195432/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGataata > 1:2415507/1‑36 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCg < 1:633869/40‑1 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCg < 1:1835638/40‑1 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCg < 1:2795762/40‑1 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCg > 1:694986/1‑67 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:2086054/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:1161899/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:424041/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:2162667/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:879121/1‑68 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:2129194/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:212754/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:2511485/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:894393/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:3339845/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1448849/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:272880/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:2790072/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:3125063/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1768943/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1799221/68‑1 (MQ=255) cATCAGATGCGTACACTCTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1126140/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:2032757/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:635931/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:1942002/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:3197607/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:851156/68‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:640067/69‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:111117/69‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:3368231/69‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:917557/69‑1 (MQ=255) | GCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTG > minE/2778345‑2778467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |