| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,778,442 | C→T | 17.9% | D129N (GAT→AAT) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,778,442 | 0 | C | T | 17.9% | 235.7 / 31.7 | 144 | D129N (GAT→AAT) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (49/69); new base T (16/10); total (65/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
ACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGT > minE/2778388‑2778510 | aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1592801/69‑1 (MQ=255)aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:49699/69‑1 (MQ=255)aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:658393/69‑1 (MQ=255)aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1200686/69‑1 (MQ=255)aCATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:750490/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:2752115/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:1590111/69‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGGCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 1:678819/69‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:1040615/68‑1 (MQ=255) cATACGCATCAGGTGAGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCa < 1:2220756/68‑1 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCg > 1:694986/1‑67 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:1161899/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:879121/1‑68 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc > 1:2162667/1‑68 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:2129194/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:3125063/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1799221/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1768943/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:3339845/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1448849/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:2511485/68‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:920125/68‑1 (MQ=255) cATCAGATGCGTACACTCTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:1126140/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:635931/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:851156/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:3197607/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:1942002/68‑1 (MQ=255) aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:2032757/68‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:111117/69‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:3368231/69‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTg < 1:917557/69‑1 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGc > 1:3249861/2‑41 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCg > 1:2531788/2‑68 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:412247/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:3206084/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:2192541/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:1449812/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:1976076/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:1869694/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:485216/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:1739069/2‑69 (MQ=255) agTACACTGTACCTGGTCGATAATAATCGCTTCGTGATTGCTTTGATCAAGCACCGCCATATTGACCGt > 1:3421084/2‑69 (MQ=255) tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAg > 1:1590332/1‑68 (MQ=255) tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGa > 1:2676854/1‑69 (MQ=255) gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCa > 1:3082108/1‑61 (MQ=255) gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCa > 1:2541928/1‑61 (MQ=255) gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCa > 1:2046168/1‑61 (MQ=255) gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCa > 1:3427711/1‑61 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:532143/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2597610/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:351432/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:792213/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2728448/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2690723/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2682864/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:1052340/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:1359569/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:186013/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2326115/67‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc < 1:2403048/67‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:35564/69‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:1615721/69‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:1819302/69‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:453174/69‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:1994065/69‑1 (MQ=255) taataaTCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:2630680/69‑1 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:889658/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:735249/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:662728/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:1815709/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:625992/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:2668601/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:2783100/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:1854491/68‑1 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACAGTTTCGCCAGAc > 1:45528/1‑58 (MQ=255) aataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATAGACCGTTTCGCCAGAc > 1:2183196/1‑58 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:1866312/1‑69 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:2664628/1‑69 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:1351625/1‑69 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:2772297/1‑69 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:2854711/1‑69 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc > 1:2138519/1‑69 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:366082/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:1059048/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:1135715/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:1261425/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:2303434/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:2173432/69‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAg < 1:3263330/69‑1 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:2968024/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:1934006/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:2927735/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:1700520/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:2767950/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:527977/1‑69 (MQ=255) aTCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAgg > 1:296982/1‑69 (MQ=255) cGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGt < 1:2453583/69‑1 (MQ=255) cGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGt < 1:2160511/69‑1 (MQ=255) cGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGt < 1:2112987/69‑1 (MQ=255) cGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGt < 1:2110823/69‑1 (MQ=255) | ACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGT > minE/2778388‑2778510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |