| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,778,836 | T→C | 47.9% | intergenic (‑10/‑190) | iclR ← / → metH | DNA‑binding transcriptional repressor/homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,778,836 | 0 | T | C | 47.9% | 0.8 / 87.0 | 96 | intergenic (‑10/‑190) | iclR/metH | DNA‑binding transcriptional repressor/homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (39/11); new base C (31/15); total (70/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTG > minE/2778775‑2778900 | cGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTc > 1:172724/1‑68 (MQ=255)cGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTc > 1:3388658/1‑68 (MQ=255) ggCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTa > 1:3266926/1‑68 (MQ=255) ggCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTAt > 1:3102275/1‑69 (MQ=255) aCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGt > 1:1161893/1‑68 (MQ=255) cggGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa < 1:2214773/69‑1 (MQ=255) cggGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa < 1:2946162/69‑1 (MQ=255) cggGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa < 1:3029663/69‑1 (MQ=255) cggGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa < 1:1478114/69‑1 (MQ=255) cggGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa < 1:2889504/69‑1 (MQ=255) ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat > 1:3402905/1‑69 (MQ=255) ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat > 1:3355568/1‑69 (MQ=255) ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat > 1:739782/1‑69 (MQ=255) ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat > 1:2727181/1‑69 (MQ=255) ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcat > 1:1859999/1‑69 (MQ=255) gcgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATcattttcatttt > 1:3379854/1‑69 (MQ=255) gcgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATcattttcatttt < 1:3298451/69‑1 (MQ=255) gcgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATcatttacatttt > 1:78664/1‑69 (MQ=255) gAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCt < 1:1600223/69‑1 (MQ=255) ggTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa < 1:926790/66‑1 (MQ=255) ggTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa < 1:516005/66‑1 (MQ=255) ggTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc > 1:2570066/1‑69 (MQ=255) tGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCaa < 1:2484115/69‑1 (MQ=255) cGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg > 1:786392/1‑58 (MQ=255) cGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg > 1:805351/1‑58 (MQ=255) cGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg > 1:931254/1‑58 (MQ=255) cGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg > 1:2090928/1‑58 (MQ=255) aCCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt < 1:388947/69‑1 (MQ=255) aCCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt < 1:491336/69‑1 (MQ=255) aCCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt < 1:1105203/69‑1 (MQ=255) aCCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt < 1:3388204/69‑1 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcat > 1:1488152/1‑37 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:2941357/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:3410102/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:2606724/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:2466433/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:3227991/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:2176657/1‑50 (MQ=255) ccATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:1980637/1‑50 (MQ=255) ccATGAAAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtta > 1:438776/1‑50 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATg > 1:367640/1‑60 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:529212/1‑64 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:3183215/1‑64 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:1338195/1‑64 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:876880/1‑64 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:78372/1‑64 (MQ=255) cATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:1593337/1‑64 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1464950/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1643499/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1276280/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1459420/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:3133400/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1354082/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1732075/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:1117639/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:2575579/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:2542438/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:923748/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:2463621/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttc > 1:2352309/1‑40 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatttta > 1:3032190/1‑39 (MQ=39) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:2963506/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:335614/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:3281778/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:1973380/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:685180/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:491728/1‑69 (MQ=255) aCAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga > 1:362854/1‑69 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:734388/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:686187/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:3128876/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:3330516/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:2267749/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:1902011/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:3160179/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:2926381/69‑2 (MQ=255) gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTATCAGCGAATGCAATAGTTgctgaaca < 1:1627075/69‑2 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt > 1:1766261/1‑58 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt > 1:2698569/1‑58 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt > 1:1350939/1‑58 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:3345774/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:270572/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:266785/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:260735/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:91594/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:3194883/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg < 1:2003215/68‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:2080812/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:1320108/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:359990/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:3280720/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:2613283/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:2489297/64‑1 (MQ=255) ctctTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga < 1:2253817/64‑1 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:3146506/1‑52 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt > 1:1151318/1‑52 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATa > 1:1623500/1‑53 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATa > 1:1798204/1‑53 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATa > 1:2545297/1‑53 (MQ=255) ttttttCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATa > 1:1035818/1‑53 (MQ=255) | CGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTG > minE/2778775‑2778900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |