| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,780,711 | A→T | 20.0% | V562V (GTA→GTT) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,780,711 | 0 | A | T | 20.0% | 90.9 / 16.0 | 50 | V562V (GTA→GTT) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (32/8); new base T (5/5); total (37/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTC > minE/2780647‑2780777 | aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1076592/1‑69 (MQ=255)aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2864585/1‑69 (MQ=255)aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1044215/1‑69 (MQ=255)aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2511895/1‑69 (MQ=255)aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2342857/1‑69 (MQ=255)aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1318247/1‑69 (MQ=255) cTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:633209/1‑68 (MQ=255) gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:3140688/1‑69 (MQ=255) gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:370677/1‑69 (MQ=255) gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:2073586/1‑69 (MQ=255) gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:3175312/1‑69 (MQ=255) gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:1694831/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGGGTATCTAACGTTTCTTTCTCGTTcc > 1:224672/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1930512/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:19044/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:3426869/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1262110/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:2925311/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1649691/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:259632/1‑69 (MQ=255) aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:2654285/1‑69 (MQ=255) tCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCa < 1:554944/69‑1 (MQ=255) aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCt > 1:2839176/1‑42 (MQ=255) aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:1553536/1‑69 (MQ=255) aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:3007848/1‑69 (MQ=255) aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:2388940/1‑69 (MQ=255) aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:2115145/1‑69 (MQ=255) gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:1229258/67‑1 (MQ=255) gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:517945/67‑1 (MQ=255) gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:2103134/67‑1 (MQ=255) gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:646523/67‑1 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:699103/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:376749/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:268059/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3299863/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3255811/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3253766/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3028480/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:2461658/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:2383541/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:198392/1‑59 (MQ=255) gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:1123011/1‑59 (MQ=255) ggcgTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:2005430/40‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCTTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:3135273/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:3258733/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:2771015/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:1996381/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:68335/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:939471/69‑1 (MQ=255) gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:992675/69‑1 (MQ=255) | ACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTC > minE/2780647‑2780777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |