| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,780,768 | A→G | 22.3% | A581A (GCA→GCG) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,780,768 | 0 | A | G | 22.3% | 86.6 / 18.7 | 67 | A581A (GCA→GCG) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (17/35); new base G (4/11); total (21/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGATC > minE/2780709‑2780813 | gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:202547/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:119092/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:908776/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:2324775/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:3311004/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:865621/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:2212005/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:1685656/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:1706552/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:3129154/66‑1 (MQ=255)gtttCTAACGTGTCGTTCTCGTTTCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCGGTGTTCctc < 1:3417390/66‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCTTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:3135273/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:3258733/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:1996381/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:68335/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:2771015/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:939471/69‑1 (MQ=255)gTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCctc < 1:992675/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:2651895/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:110202/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:2542307/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:3286608/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:3382963/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:3408681/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:798480/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:868650/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:893037/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:93868/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:240757/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:1070948/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:1146821/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:1434707/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:1585296/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:1798058/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:2006356/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:2210375/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:2459054/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac < 1:2281706/69‑1 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctac > 1:2256021/1‑69 (MQ=255) tCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGAGAAGCCATTCACGCAGTGTTCCTctac > 1:2835969/1‑69 (MQ=255) cTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctat < 1:2943791/68‑2 (MQ=255) tAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTctacta > 1:2232641/1‑69 (MQ=255) tctttctCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTAtt > 1:3395649/1‑69 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:3221088/66‑1 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:1190182/66‑1 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:2676751/66‑1 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:500822/66‑1 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:361009/66‑1 (MQ=255) tcGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGc < 1:3281100/66‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:1884117/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:2951187/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:986982/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:2585918/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:2805546/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:920740/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:1255847/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:1315900/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:3012249/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:146032/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:2753135/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:492799/68‑1 (MQ=255) gTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATg < 1:1939421/68‑1 (MQ=255) tGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATGGa < 1:3430446/69‑1 (MQ=255) ccGGTGCGCGAAGCCATTCACGCGGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGAt > 1:1971567/1‑68 (MQ=255) ccGGTGCGCGAAGCCATTCACGCGGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGATc > 1:832483/1‑69 (MQ=255) ccGGTGCGCGAAGCCATTCACGCGGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGATc > 1:2037697/1‑69 (MQ=255) ccGGTGCGCGAAGCCATTCACGCGGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGATc > 1:1836625/1‑69 (MQ=255) | GTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATCCGGTGCGCGAAGCCATTCACGCAGTGTTCCTCTACTACGCTATTCGCAATGGCATGGATATGGGGATC > minE/2780709‑2780813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |