| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 241,084 | C→T | 42.5% | V1047M (GTG→ATG) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 241,084 | 0 | C | T | 42.5% | 29.7 / 64.2 | 87 | V1047M (GTG→ATG) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (22/28); new base T (21/16); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
TAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGG > minE/241016‑241150 | tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:1070228/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:980244/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:1161485/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:845369/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:3009110/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:2963162/1‑69 (MQ=255)tAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCAc > 1:2061174/1‑69 (MQ=255) agagATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAg < 1:3252389/69‑1 (MQ=255) agagATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAg < 1:2529938/69‑1 (MQ=255) agagATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAg < 1:2538386/69‑1 (MQ=255) agagATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAg < 1:3422236/69‑1 (MQ=255) agagATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAg < 1:485944/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1817645/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2720103/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:479375/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:190353/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1919259/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:3228755/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2281758/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:3231/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1816241/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:280635/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2824884/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:612912/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:717226/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1260393/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2899339/69‑1 (MQ=255) ttCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTg > 1:1781231/1‑69 (MQ=255) tCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTATGCTGTAGCCCAGTCCATTg < 1:514840/68‑1 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGGCGTTGAt > 1:1408251/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGa > 1:1629080/1‑68 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:1020227/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:3050543/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:2865706/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:3248476/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:856964/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:1746819/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:1778231/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:455152/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:2182684/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCACAGGCCGTTGAt > 1:3376484/1‑69 (MQ=255) aTTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATc > 1:3125601/1‑69 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACtttt < 1:2615244/67‑2 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:396518/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:3385839/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:3007322/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2987739/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:512247/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:730892/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:3239289/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:999853/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2797596/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2567791/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2318164/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2101935/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2895176/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:779620/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:3429108/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2458974/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2005819/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2200648/68‑1 (MQ=255) tAGTTATTTAACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:1859314/67‑1 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1033669/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1759204/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1765779/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1858116/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1934868/1‑69 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTccat > 1:934760/1‑40 (MQ=25) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACtt > 1:3314314/1‑57 (MQ=37) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGAt > 1:233559/1‑61 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCt > 1:92309/1‑63 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2851452/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2876781/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:89025/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1362266/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2929200/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1550864/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1609019/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:52681/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2648132/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:3071400/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:395627/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:360481/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:3206014/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2281905/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:3324311/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAc > 1:1095361/1‑67 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCAAGTCCATTGATCTTCTTCACTTTGa > 1:2030315/1‑60 (MQ=255) tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAg > 1:259199/1‑37 (MQ=255) tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCAcc > 1:1640536/1‑68 (MQ=255) tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCAcc > 1:432273/1‑68 (MQ=255) aCTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCgg < 1:2771199/68‑1 (MQ=255) aCTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCgg < 1:157309/68‑1 (MQ=255) aCTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCgg < 1:1158434/68‑1 (MQ=255) | TAACGACCAGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGG > minE/241016‑241150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |