BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,783,748	C→T	50.4%	L274L (CTG→TTG)	yjbB →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,783,748	0	C	T	50.4%	‑4.6 / 123.2	129	L274L (CTG→TTG)	yjbB	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (32/32); new base T (47/18); total (79/50)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

Only 100 of 129 total aligned reads displayed.

TGTCCATTTGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGCC  >  minE/2783696‑2783803

tGTCCATTTGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTcc                                          >  1:90856/1‑68 (MQ=255)

tGTCCATTTGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCa                                         >  1:2354561/1‑69 (MQ=255)

     aTTTGGTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:718329/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGTTCTATTTCCACGtct                                    >  1:2840513/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTAt                                              >  1:1105642/1‑59 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1573708/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:707202/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1947166/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:2681319/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:2710507/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1800897/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:2797182/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1561939/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:3255850/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:3359684/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:340482/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:87612/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1420655/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:1387172/1‑69 (MQ=255)

     aTTTGCTGGCAGAGACAATGGGGAAGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtct                                    >  1:3056151/1‑69 (MQ=255)

        tGCTGGCAGAGACATTGGGGAAGTTGTCATTGCCAAAAGCGGAACt                                                        >  1:596804/1‑46 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:1568206/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:3033816/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:2362521/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:2110315/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:2478936/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:3004658/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:2607117/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:1031810/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:2611115/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:1302306/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:3153391/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:1775721/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:481812/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtcttc                                  >  1:133473/1‑68 (MQ=255)

        tGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGtctt                                   >  1:471806/1‑67 (MQ=255)

               agagACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCt                          <  1:186117/69‑1 (MQ=255)

                gagaCAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCt                          <  1:1071823/68‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTTGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:1900771/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:2344423/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:1715347/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:781740/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:573685/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:3421663/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:1977188/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:2056047/69‑1 (MQ=255)

                       tGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:2210137/69‑1 (MQ=255)

                        ggggAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTAc                     >  1:1853281/1‑65 (MQ=255)

                        ggggAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTAc                     >  1:699489/1‑65 (MQ=255)

                        ggggAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGtt                  <  1:3276661/68‑1 (MQ=255)

                            aaGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTg             >  1:1554307/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTa                                               >  1:2337935/1‑35 (MQ=37)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:3207991/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:1105495/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:3356569/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:1403815/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:157972/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:3432911/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:1561453/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:473680/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:2905406/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:2901487/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:1718973/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:545168/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:568400/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:2636249/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:1908790/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:2171647/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGCTGCCTg             >  1:680068/1‑69 (MQ=255)

                            aaGTTGCCATTGCCAAAAGCGGAATTGGTGAGCTATTTCCACGTCTTCTACAACCTTGTACGCTGc                >  1:2395394/1‑66 (MQ=255)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:400533/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:369874/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:852957/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:856768/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:918648/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:3116731/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:1334071/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:2397919/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:2431702/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:270117/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:278483/42‑1 (MQ=39)

                                 gccATTGCCAAAAGCGGAATTGGAGATCTATTTCCACGtcttct                                 <  1:2919941/42‑1 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        >  1:1782949/1‑69 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:1237050/69‑1 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        >  1:860912/1‑69 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:794147/69‑1 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:729801/69‑1 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:2042011/69‑1 (MQ=255)

                                 gTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:2774177/69‑1 (MQ=255)

                                  ccATTGCCAAAAGCGGAATTGGTGATCTATTTCCACGtcttct                                 <  1:2211988/42‑1 (MQ=39)

                                      tGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCAt        <  1:1536150/64‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  >  1:297141/1‑69 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:2912701/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:2943878/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:2938372/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  >  1:3283767/1‑69 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:1325813/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:2721429/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:1251158/69‑1 (MQ=255)

                                       gCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGcc  <  1:1204150/69‑1 (MQ=255)

TGTCCATTTGCTGGCAGAGACAATGGGGAAGTTGTCATTGCCAAAAGCGGAACTGGTGATCTATTTCCACGTCTTCTACAACCTTGTACGTTGCCTGGTCATGCTGCC  >  minE/2783696‑2783803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`