BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,784,005	T→C	46.4%	G359G (GGT→GGC)	yjbB →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,784,005	0	T	C	46.4%	11.2 / 107.2	123	G359G (GGT→GGC)	yjbB	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (40/26); new base C (29/28); total (69/54)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

Only 100 of 123 total aligned reads displayed.

TGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCC  >  minE/2783947‑2784068

tGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGc                                                       >  1:2052988/1‑69 (MQ=255)

tGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGc                                                       >  1:406689/1‑69 (MQ=255)

        ggCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGgaga                                                 >  1:3016212/1‑67 (MQ=255)

        ggCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGgaga                                                 >  1:2703075/1‑67 (MQ=255)

        ggCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGgaga                                                 >  1:1904561/1‑67 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:2671603/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:1768273/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:1656215/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:3204831/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:3246363/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:3281306/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:1945565/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:3374259/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:2861411/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:2316230/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:2442475/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:607964/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAaga                                             >  1:2806577/1‑69 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGCGAGCCACGGCAGGAGAAag                                              >  1:2803248/1‑68 (MQ=255)

          cGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGAACGGCGAGCCACGGCAGGAGAAaga                                             >  1:1934661/1‑69 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:3104749/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:2858108/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:1017246/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:3182339/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:1162732/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:2454187/1‑49 (MQ=255)

              gCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGc                                                             >  1:590801/1‑49 (MQ=255)

                   ggAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTaa                                    >  1:1878713/1‑69 (MQ=255)

                   ggAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTaa                                    >  1:1494664/1‑69 (MQ=255)

                    gAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAg                                   >  1:2781316/1‑69 (MQ=255)

                    gAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAg                                   >  1:1952970/1‑69 (MQ=255)

                    gAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAg                                   >  1:10120/1‑69 (MQ=255)

                    gAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAg                                   >  1:1652052/1‑69 (MQ=255)

                       cAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:194600/68‑1 (MQ=255)

                            gatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCt                                          >  1:2411270/1‑54 (MQ=255)

                            gatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAg                            >  1:2640849/1‑68 (MQ=255)

                            gatgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAg                            >  1:860648/1‑68 (MQ=255)

                             atgGAAGGTTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:2952630/69‑1 (MQ=255)

                             atgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:1690110/69‑1 (MQ=255)

                             atgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:1479816/69‑1 (MQ=255)

                             atgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:3349422/69‑1 (MQ=255)

                             atgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:2090541/69‑1 (MQ=255)

                             atgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:538622/69‑1 (MQ=255)

                              tgGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:684827/68‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:3141601/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:1088116/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:1147817/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:747652/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:1271774/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:2237428/60‑1 (MQ=255)

                                      ttGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          >  1:1620055/1‑60 (MQ=255)

                                       tGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                          <  1:2810312/59‑1 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:601003/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:342637/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2919725/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:3417822/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:3125235/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:862378/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1013319/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1733073/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1834567/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1846991/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1498445/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1459657/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1372181/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2191388/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1301031/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2354822/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2481027/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1170552/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1148610/53‑2 (MQ=255)

                                          aTAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2602781/53‑2 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:439524/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:3402541/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:1423860/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:1098427/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:2605233/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:1565660/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:267707/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:1717894/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:2532784/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:1614744/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:3014786/1‑68 (MQ=255)

                                           tAAAGTGATGCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCTGATGACATCAACGTtc             >  1:2062083/1‑68 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:374262/46‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:2736724/46‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:1351949/46‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:3070061/46‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:3239747/46‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGTCAGATGATATCAACGTtctc           >  1:3300407/1‑68 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgattat                       <  1:1872340/56‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgatgat                       <  1:1227130/56‑1 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTtctc           >  1:222515/1‑68 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTtctc           >  1:1250453/1‑68 (MQ=255)

                                             aaGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTtctc           >  1:2784118/1‑68 (MQ=255)

                                                   tGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                 <  1:1301790/40‑1 (MQ=255)

                                                    gCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:2104976/43‑2 (MQ=255)

                                                    gCACGGCGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGct                             <  1:1351780/43‑2 (MQ=255)

                                                     cACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGcc  >  1:283356/1‑69 (MQ=255)

                                                     cACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGcc  >  1:3274937/1‑69 (MQ=255)

TGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCC  >  minE/2783947‑2784068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`