Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,784,311 G→T 17.1% V461V (GTG→GTT)  yjbB → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,784,3110GT17.1% 185.3 / 21.8 87V461V (GTG→GTT) yjbBpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (48/24);  new base T (10/5);  total (58/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAA  >  minE/2784245‑2784379
                                                                  |                                                                    
tGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGtt                                                                    >  1:3323871/1‑69 (MQ=255)
tGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGtt                                                                    >  1:2377066/1‑69 (MQ=255)
tGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGtt                                                                    >  1:2224311/1‑69 (MQ=255)
  ggTTGAAGGATCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:1076280/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:317347/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:1185251/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:538232/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:68426/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:234528/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:711762/1‑69 (MQ=255)
  ggTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                                                  >  1:746232/1‑69 (MQ=255)
          gAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGc                                                          <  1:641842/69‑1 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCg                                                         >  1:374408/1‑68 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:822832/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:1411484/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:2579267/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:2222518/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:2670836/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:2717133/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:2176185/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:716479/1‑69 (MQ=255)
            aCTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGa                                                        >  1:3285642/1‑69 (MQ=255)
             cTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGAt                                                       <  1:1757952/69‑1 (MQ=255)
             cTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGAt                                                       <  1:2039834/69‑1 (MQ=255)
                gATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTc                                                    >  1:433400/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCa                                                   >  1:56505/1‑59 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:1614032/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:3364262/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:2359785/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:703565/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:2294727/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:495164/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:943835/1‑69 (MQ=255)
                           tGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcg                                         >  1:1725251/1‑69 (MQ=255)
                             aGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAGCAGCGCtcgtc                                       >  1:1258488/1‑69 (MQ=255)
                             aGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtc                                       >  1:3323815/1‑69 (MQ=255)
                             aGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtc                                       >  1:1754225/1‑69 (MQ=255)
                             aGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtc                                       >  1:1876776/1‑69 (MQ=255)
                             aGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtc                                       >  1:1411459/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:1607761/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:2226463/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:295525/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:2319599/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:1334527/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:2525383/54‑1 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtc                                          >  1:2461561/1‑62 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:2480567/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:3232537/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:3351931/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:1982197/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:2023649/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:1383914/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:2700889/1‑69 (MQ=255)
                                 aTTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt                                   >  1:498922/1‑69 (MQ=255)
                                 aTTCCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTgg                                                           >  1:2669794/1‑45 (MQ=255)
                                  ttGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAc                                                  <  1:2958638/53‑1 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtc                                       >  1:2728922/1‑54 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        <  1:1617347/69‑1 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        <  1:207578/69‑1 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        >  1:3035734/1‑69 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        >  1:1304763/1‑69 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        >  1:2969832/1‑69 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagca                        >  1:2529477/1‑69 (MQ=255)
                                            aTTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCgcagc                         >  1:2190521/1‑68 (MQ=255)
                                            aTTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGATGTTACCAGCGCCCGTCGTTTGCGCCgcagca                        <  1:33266/69‑1 (MQ=255)
                                            aTTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGATGTTACCAGCGCCCGTCGTTTGCGCCgcagca                        <  1:2501763/69‑1 (MQ=255)
                                            aTTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGATGTTACCAGCGCCCGTCGTTTGCGCCgcagca                        <  1:2393913/69‑1 (MQ=255)
                                            aTTTAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGATGTTACCAGCGCCCGTCGTTTGCGCCgcagca                        <  1:2189716/69‑1 (MQ=255)
                                             tttAAAACTGGCAATGTCGGTTTTCTTCTCTGGCGATGTTACCAGCGCCCGTCGTTTGCGCCgaagca                        <  1:1142739/68‑1 (MQ=255)
                                               tAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      >  1:1717158/1‑68 (MQ=255)
                                               tAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAAc                     >  1:1330718/1‑69 (MQ=255)
                                               tAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAAc                     >  1:128765/1‑69 (MQ=255)
                                               tAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAAc                     >  1:2622039/1‑69 (MQ=255)
                                                         aaTGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCg           <  1:3079709/69‑1 (MQ=255)
                                                            gTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCAt        >  1:2852356/1‑69 (MQ=255)
                                                            gTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCAt        >  1:1404135/1‑69 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:77209/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:991458/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:3018381/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:2753865/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:362838/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:261968/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:2563779/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:1153174/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:1918026/52‑1 (MQ=255)
                                                               ggTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:1828448/52‑1 (MQ=255)
                                                               ggTGTGCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCaaa                      <  1:734459/52‑1 (MQ=255)
                                                                 tgtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTa   <  1:644006/69‑1 (MQ=255)
                                                                 tgtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTa   <  1:2386683/69‑1 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:1095183/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:1427975/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:1545707/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:1842973/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:2333336/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:2475279/1‑69 (MQ=255)
                                                                  gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTaa  >  1:2653732/1‑69 (MQ=255)
                                                                  |                                                                    
TGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAA  >  minE/2784245‑2784379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑