| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,785,363 | T→A | 34.4% | intergenic (‑23/‑189) | pepE ← / → yjbC | (alpha)‑aspartyl dipeptidase/23S rRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,785,363 | 0 | T | A | 34.4% | 90.0 / 75.6 | 96 | intergenic (‑23/‑189) | pepE/yjbC | (alpha)‑aspartyl dipeptidase/23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base T (39/24); new base A (7/26); total (46/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG > minE/2785298‑2785422 | cTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc > 1:190293/1‑69 (MQ=255)cTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc > 1:1261075/1‑69 (MQ=255)cTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc > 1:264942/1‑69 (MQ=255)cTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc > 1:287695/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:28040/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:1596166/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:1694289/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:1821415/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:1404784/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:2881242/1‑69 (MQ=255) cGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCa > 1:754565/1‑69 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:923988/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:2863566/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:2569793/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:2025733/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:2899697/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:3189562/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:547865/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa > 1:1153526/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAAGCa > 1:256545/1‑60 (MQ=255) gAGTTACTCAATAAAAGCAGTTCCATTACTTCTACGGTTAGTAAGAAATCACGGGAGGCa > 1:2854424/1‑60 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:2428312/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:2082435/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:342175/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:435346/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:536579/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:1173568/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:2588043/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:712364/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:738501/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:898188/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:2623314/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:902913/68‑1 (MQ=255) tACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:1596985/68‑1 (MQ=255) aCTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:1309382/67‑1 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAttt < 1:3364338/67‑1 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAttt < 1:2146274/67‑1 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAttt < 1:58811/67‑1 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:3197912/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:222204/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:2980364/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:2939806/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:2140679/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:2476939/1‑68 (MQ=255) tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTa > 1:396744/1‑68 (MQ=255) aaaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAc < 1:1334038/69‑1 (MQ=255) aaaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAc < 1:2744240/69‑1 (MQ=255) gCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt < 1:1938312/69‑1 (MQ=255) gCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt < 1:181585/69‑1 (MQ=255) ccATTACTTCTCCGGTTATTAAGAAATCACGGCAGGc < 1:1587684/37‑1 (MQ=38) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:2110433/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:1944875/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:3110762/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:606509/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:2833082/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:2582591/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:2245571/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:1933908/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:617713/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:610454/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc < 1:2416821/37‑1 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGa > 1:1457854/1‑69 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGa > 1:1248816/1‑69 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGa > 1:283254/1‑69 (MQ=255) ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGa > 1:279287/1‑69 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:19261/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:879560/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:57587/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:570192/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:525080/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:2024780/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:347342/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:181432/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:2769243/69‑1 (MQ=255) tACTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:1291568/69‑1 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt > 1:3286308/1‑58 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt > 1:1956776/1‑58 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt > 1:828423/1‑58 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATgg > 1:1987999/1‑69 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATgg > 1:2654337/1‑69 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATgg > 1:1530167/1‑69 (MQ=255) aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATgg > 1:829811/1‑69 (MQ=255) aCTTCTCCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGCGAATTTAACCACTTACTGACATg < 1:1505747/68‑1 (MQ=255) tcCGGTTAGTAAGAAAACACGGAAGGAATGCTACCACTGc < 1:1419786/40‑1 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCAc < 1:2153913/63‑1 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCAc < 1:110267/63‑1 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCAc < 1:3033807/63‑1 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCAc < 1:3155152/63‑1 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGt > 1:2868257/1‑68 (MQ=255) tAGTAAGAAATCACGGGAGGCATGCTACCACCGCGAATTTAACCACTTACTGACATGGCTCAc < 1:476644/63‑1 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:1281604/1‑69 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:1429317/1‑69 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:3162397/1‑69 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:3172122/1‑69 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:1982243/1‑69 (MQ=255) aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg > 1:1228954/1‑69 (MQ=255) | CTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG > minE/2785298‑2785422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |