| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,785,788 | A→C | 32.9% | V79V (GTA→GTC) | yjbC → | 23S rRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,785,788 | 0 | A | C | 32.9% | 58.0 / 54.8 | 73 | V79V (GTA→GTC) | yjbC | 23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base A (24/25); new base C (21/3); total (45/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
TTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCGTGT > minE/2785723‑2785852 | ttgattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGc < 1:1584315/69‑1 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTa > 1:426872/1‑64 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTa > 1:1521659/1‑64 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac < 1:2227778/69‑1 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac < 1:2323389/69‑1 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac < 1:1767847/69‑1 (MQ=255) gattgaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac < 1:2562653/69‑1 (MQ=255) gaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac > 1:3351117/1‑68 (MQ=255) gaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac > 1:1214044/1‑68 (MQ=255) gaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac > 1:942451/1‑68 (MQ=255) gaACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac > 1:2608818/1‑68 (MQ=255) ccTCGTGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:1739771/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:159812/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:2616796/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:1687823/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:2681996/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:2830762/69‑1 (MQ=255) ccTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:3017630/69‑1 (MQ=255) cTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:1290272/68‑1 (MQ=255) tGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac < 1:2862656/43‑1 (MQ=255) tGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAAc < 1:3275101/68‑1 (MQ=255) tATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac > 1:2393826/1‑39 (MQ=255) tATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa < 1:1663428/43‑1 (MQ=255) tATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTc > 1:55721/1‑67 (MQ=255) tATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTc > 1:370266/1‑67 (MQ=255) tATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTc > 1:1917950/1‑67 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:606010/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:3430934/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:847955/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:1747717/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:1445491/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:127114/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:438857/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:1233715/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:2094155/1‑42 (MQ=255) aTCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGAt > 1:1213961/1‑69 (MQ=255) aTCGACCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGaa > 1:2905320/1‑42 (MQ=255) cTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGtt < 1:2446178/69‑1 (MQ=255) cTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGtt < 1:2714635/69‑1 (MQ=255) aaCAAGCCGGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTa < 1:1690489/67‑1 (MQ=255) cAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAAc < 1:1899039/67‑1 (MQ=255) ccGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGct < 1:349813/69‑2 (MQ=255) ccGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa < 1:1988097/69‑1 (MQ=255) ccGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa < 1:595345/69‑1 (MQ=255) ccGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa < 1:2163236/69‑1 (MQ=255) ccGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa < 1:498957/69‑1 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:87665/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:369363/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:785975/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2468416/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:961727/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:3039264/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1832416/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2677096/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2673105/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1091253/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2450322/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2362168/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1836584/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1904312/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:2109430/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1571065/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:1977943/1‑69 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa > 1:1739530/1‑68 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa > 1:73886/1‑68 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa > 1:578133/1‑68 (MQ=255) cGTCGGTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCa > 1:424997/1‑68 (MQ=255) cGTCGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCaa > 1:3281411/1‑69 (MQ=255) ggTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAAcgc < 1:592724/69‑1 (MQ=255) ggTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAAcgc < 1:478619/69‑1 (MQ=255) ggTATTGTCAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAAcgc < 1:144632/69‑1 (MQ=255) ttGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCgtgt > 1:1134124/1‑69 (MQ=255) ttGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCgtgt > 1:542488/1‑69 (MQ=255) ttGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCgtgt > 1:2311684/1‑69 (MQ=255) ttGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCgtgt > 1:3369731/1‑69 (MQ=255) | TTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCGAGCGCGATAACATTGTCGATTTCGTTAACCACAGCAAACGCGTGT > minE/2785723‑2785852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |