| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,790,362 | C→T | 45.4% | T469T (ACC→ACT) | pgi → | glucosephosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,790,362 | 0 | C | T | 45.4% | 20.4 / 79.7 | 99 | T469T (ACC→ACT) | pgi | glucosephosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (18/36); new base T (30/15); total (48/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAA > minE/2790298‑2790430 | gTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAAc > 1:1811460/1‑68 (MQ=255)gTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAAc > 1:2411582/1‑68 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACt > 1:491264/1‑68 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:2273335/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:3162159/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:2832866/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:1392192/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:1241072/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:2230343/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:1143218/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:1631713/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:1138935/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTGTTCGAAGGTAACCGCCCGACTAACTc > 1:2945126/1‑69 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:2386668/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:400420/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:2382691/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:3239682/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:800049/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:2985445/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:2844644/69‑1 (MQ=255) tAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:1556962/69‑1 (MQ=255) aaaGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTc < 1:2800227/68‑1 (MQ=255) aaGATCCGGCAACGGTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCa < 1:2611067/69‑1 (MQ=255) aaGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCa < 1:1906265/69‑1 (MQ=255) gATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCAt < 1:876045/68‑1 (MQ=255) aaCGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCtgctgc > 1:1187133/1‑68 (MQ=255) cTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATc < 1:1937384/67‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCTCTCCGTTCAg < 1:2949598/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:2594002/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:2865596/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:2914337/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:2643113/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1926546/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1686105/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:2918942/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1021994/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:695260/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:673495/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1286199/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1847772/69‑1 (MQ=255) gCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1884267/69‑1 (MQ=255) ccGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:1140291/68‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:2889180/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:1609013/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:1575916/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:2974171/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:3170899/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:125492/69‑1 (MQ=255) ttCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg < 1:2567775/69‑1 (MQ=255) cAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCg > 1:3284749/1‑58 (MQ=255) cAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTggg > 1:2159632/1‑69 (MQ=255) cAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTggg > 1:2922884/1‑69 (MQ=255) cAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTgg > 1:2100312/1‑68 (MQ=255) tATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCg > 1:2028366/1‑68 (MQ=255) ttCGAAGGTAACCGCCCTACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:878137/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGGTGCGTGAAATCACTCCGTTCAGCCTg > 1:2306701/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:1055148/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:2798197/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:2382710/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:742515/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:2374671/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:1150437/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:1356773/1‑60 (MQ=255) ttCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTg > 1:1751768/1‑60 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:1514289/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:2653288/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:1479697/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:3407158/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:360498/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:1287757/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:525573/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:867830/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:1528297/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:2715926/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:2828328/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:2032748/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:1682289/68‑1 (MQ=255) cGAAGGTAACCGCCCGACTAACTACATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:2992653/68‑1 (MQ=255) gAAGGTAACCGCCCGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGa < 1:174151/67‑1 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGa > 1:1085400/1‑68 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGa > 1:313806/1‑68 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:1321989/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:2644766/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:1113098/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:678762/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:1148205/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:662340/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:594997/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:552206/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:537477/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:2172597/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:1976592/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:379195/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:370963/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:1876611/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:3414370/1‑69 (MQ=255) ccGACTAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAg > 1:2959051/1‑69 (MQ=255) cGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAg < 1:3005031/40‑1 (MQ=255) cAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACaa > 1:1509616/1‑69 (MQ=255) cAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACaa > 1:1037206/1‑69 (MQ=255) | GTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAA > minE/2790298‑2790430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |