| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,791,709 | G→A | 40.2% | L110L (TTG→TTA) | ubiC → | chorismate pyruvate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,791,709 | 0 | G | A | 40.2% | 31.4 / 49.0 | 72 | L110L (TTG→TTA) | ubiC | chorismate pyruvate lyase |
| Reads supporting (aligned to +/- strand): ref base G (5/38); new base A (8/21); total (13/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAT > minE/2791643‑2791774 | gCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTgg > 1:2530782/1‑68 (MQ=255)gCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTgg > 1:748661/1‑68 (MQ=255)gCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTgg > 1:743566/1‑68 (MQ=255)gCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTg > 1:1210602/1‑67 (MQ=255) tGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTaa < 1:839510/69‑1 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:1032780/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:3364245/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:2088731/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:2073543/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:1851536/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:1734473/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGt > 1:1552584/1‑62 (MQ=255) gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTAGCGTTACAAAAATTAGGt > 1:1360361/1‑62 (MQ=255) cgtcgtTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAgg > 1:2907967/1‑69 (MQ=255) cgtcgtTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAg > 1:1651599/1‑68 (MQ=255) gtTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGc < 1:3003232/69‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:1533092/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:3129499/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:3228171/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2268479/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:3288728/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2151708/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2136725/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:97562/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2030412/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:654472/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:1333965/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:1865459/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:1919706/68‑1 (MQ=255) tCCTGTGTCAACGTTAAGCGGGCCGCAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2264955/68‑1 (MQ=255) ccTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:1317682/67‑1 (MQ=255) ccTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:2488158/67‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:1982855/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:3251135/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:1305196/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:2092190/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:199309/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:987768/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:2698087/41‑1 (MQ=255) aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:1522125/41‑1 (MQ=255) gTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCAcatc < 1:1754085/69‑1 (MQ=255) gTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTAGGTa < 1:2320803/38‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:3286551/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:3305299/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:3034828/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:998066/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:1106183/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:2394041/68‑1 (MQ=255) cGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGa < 1:2252739/68‑1 (MQ=255) gCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTAACATCATCGa < 1:3215656/65‑1 (MQ=255) cGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCATTAGGACGCTATCTGTTCACATCATCGACATTaa < 1:654113/69‑1 (MQ=255) gCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAAccc > 1:422522/1‑68 (MQ=255) tGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCggg < 1:139049/69‑1 (MQ=255) tGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCggg < 1:1708971/69‑1 (MQ=255) gCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCt < 1:3238001/37‑1 (MQ=255) aCAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTa > 1:3391234/1‑68 (MQ=255) aCAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTa > 1:2748882/1‑68 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:2232857/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:417767/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:784951/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:648953/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:3016579/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:153252/69‑1 (MQ=255) aaaaTTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTga < 1:2865740/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2987168/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2256200/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:1179749/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:1311470/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2992280/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:714136/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:1805654/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:509896/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:293678/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2129805/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2135451/69‑1 (MQ=255) aTTAGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAt < 1:2297144/69‑1 (MQ=255) | GCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGAT > minE/2791643‑2791774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |