| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,794,549 | A→G | 24.9% | I264I (ATT→ATC) | plsB ← | glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,794,549 | 0 | A | G | 24.9% | 82.9 / 24.7 | 72 | I264I (ATT→ATC) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (27/27); new base G (12/6); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTT > minE/2794482‑2794616 | cGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa < 1:2553430/68‑1 (MQ=255)cGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa < 1:63872/68‑1 (MQ=255) aTGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat > 1:2145913/1‑69 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2470354/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2425977/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1138805/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:815590/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:124792/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2413908/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2262579/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1457447/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:3407683/69‑1 (MQ=255) cGTTGATGCCCTGGTAAAGTCGGTTCCAGATGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:954093/69‑1 (MQ=255) ttGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1075547/67‑1 (MQ=255) gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:2147960/68‑1 (MQ=255) gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:3111888/68‑1 (MQ=255) gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:41916/68‑1 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa > 1:1491695/1‑62 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:1863487/1‑68 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:1313854/1‑68 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:3093979/1‑68 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:3316383/1‑68 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:2216407/1‑68 (MQ=255) tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:762632/1‑68 (MQ=255) aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAGTCATCTCGTAAGAGAAATTagcc > 1:396035/1‑68 (MQ=255) aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgcc > 1:1283079/1‑68 (MQ=255) aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgcc > 1:2136913/1‑68 (MQ=255) tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:1878168/69‑1 (MQ=255) tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:255610/69‑1 (MQ=255) tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:251533/69‑1 (MQ=255) ggTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2653467/48‑1 (MQ=255) ggTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:230474/41‑1 (MQ=255) ggTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt < 1:3127829/69‑1 (MQ=255) tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:370626/1‑69 (MQ=255) tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:1823862/1‑69 (MQ=255) tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:1544298/1‑69 (MQ=255) aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:2493952/69‑1 (MQ=255) aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:49226/69‑1 (MQ=255) aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg > 1:3202963/1‑69 (MQ=255) aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:1720096/69‑1 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:82901/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3182644/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:2514196/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3266505/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:1748501/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTTCATCAGCGCAATCGCGTTctgc > 1:2470639/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCTCGTTctgc > 1:464302/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2990191/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:100190/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2286494/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:438512/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2257310/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2166920/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:3386694/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1968414/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1925577/1‑69 (MQ=255) tcagtaagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATc > 1:1294529/1‑51 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1868386/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1179197/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:733123/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1867776/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3222560/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3166649/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTgcgc > 1:2348052/1‑69 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1669036/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:595794/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:165557/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:71310/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:2503181/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1019032/68‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:2856228/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:650296/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:266845/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:1057630/69‑1 (MQ=255) | CGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTT > minE/2794482‑2794616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |