| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,794,591 | T→C | 36.7% | A250A (GCA→GCG) | plsB ← | glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,794,591 | 0 | T | C | 36.7% | 57.1 / 61.2 | 98 | A250A (GCA→GCG) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (39/23); new base C (29/7); total (68/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
ATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT > minE/2794532‑2794657 | aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:2493952/69‑1 (MQ=255)aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg > 1:3202963/1‑69 (MQ=255)aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:1720096/69‑1 (MQ=255)aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:49226/69‑1 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3266505/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:1748501/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3182644/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:82901/1‑69 (MQ=255) cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:2514196/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTTCATCAGCGCAATCGCGTTctgc > 1:2470639/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCTCGTTctgc > 1:464302/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2257310/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:100190/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:3386694/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:438512/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2990191/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1925577/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1968414/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2166920/1‑69 (MQ=255) tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2286494/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1868386/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1867776/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3166649/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3222560/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:733123/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1179197/1‑69 (MQ=255) cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTgcgc > 1:2348052/1‑69 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:71310/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1019032/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:2503181/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:595794/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1669036/68‑1 (MQ=255) gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:165557/68‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:650296/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:1057630/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:266845/69‑1 (MQ=255) gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:2856228/69‑1 (MQ=255) gatcatcTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1517126/64‑1 (MQ=255) tcatcTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:3316750/50‑1 (MQ=255) tcatcTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAt > 1:1040728/1‑69 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:2283768/69‑1 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:428604/69‑1 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:727560/69‑1 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:3354468/69‑1 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:3081675/69‑1 (MQ=255) agaAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:2073019/69‑1 (MQ=255) aaaTTCGCCGCAATCTCTTCCATCAGTGCAATCGCGt > 1:1184999/1‑37 (MQ=255) aaaTTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtg > 1:1298779/1‑69 (MQ=255) aaTTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgc < 1:2496614/69‑1 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:55422/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:1462056/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:2090637/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:1441733/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:519148/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:521480/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:544321/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:2354762/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:2822695/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:419923/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:1700829/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:1044680/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:1030726/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:885602/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:415874/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:906925/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttttttt > 1:3402877/1‑64 (MQ=255) tCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgcttg > 1:1658818/1‑67 (MQ=255) cAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCtt < 1:598250/69‑1 (MQ=255) cAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCtt < 1:2462645/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCTCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:2468274/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:1331692/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:3405799/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:91018/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:2862974/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:1685083/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:1783491/1‑65 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:2523958/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:142120/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:2714145/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:3229517/69‑1 (MQ=255) aTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa < 1:1677107/69‑1 (MQ=255) aTCACTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcgc > 1:2642429/1‑65 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag > 1:2942785/1‑36 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAttt > 1:1518993/1‑40 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2513534/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2476721/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2377471/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:1069959/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:1207787/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:1617202/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2755309/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2811310/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:5504/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2342622/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:2035825/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:1480693/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:416236/1‑69 (MQ=255) aGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt > 1:1568869/1‑69 (MQ=255) tGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtttttt < 1:2952126/41‑1 (MQ=255) | ATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT > minE/2794532‑2794657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |