| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,795,002 | A→G | 37.2% | R113R (CGT→CGC) | plsB ← | glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,795,002 | 0 | A | G | 37.2% | 21.0 / 24.5 | 43 | R113R (CGT→CGC) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (17/10); new base G (7/9); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
GCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCG > minE/2794938‑2795054 | gCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACgg < 1:1439501/68‑1 (MQ=255) cgcgACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga < 1:2881599/69‑1 (MQ=255) gcgACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga < 1:1329716/68‑1 (MQ=255) gcgACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga < 1:2185980/68‑1 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:1026679/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:723105/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:662286/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:647822/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:606411/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:583444/1‑69 (MQ=255) aaaCATCACCGATACAGGCACCATCTGCACATCCAGATTTGGGTTGCTGCGGTGCAAATCGAGATAGTc > 1:1023091/1‑69 (MQ=255) acTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa < 1:2883423/69‑1 (MQ=255) ggCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATa > 1:1524510/1‑69 (MQ=255) aCCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGa < 1:914362/68‑1 (MQ=255) cATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc < 1:954128/69‑1 (MQ=255) aTCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc < 1:1190734/68‑1 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGa > 1:906074/1‑49 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:2166989/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:73809/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:2880894/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:2093799/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:1763832/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:2926163/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:1861612/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:567851/1‑69 (MQ=255) tGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct > 1:407555/1‑69 (MQ=255) cacaTCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAg < 1:2228424/51‑1 (MQ=255) tCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt > 1:2436568/1‑65 (MQ=255) tCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg > 1:514240/1‑68 (MQ=255) tCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGc > 1:89222/1‑69 (MQ=255) cAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg > 1:422816/1‑68 (MQ=255) cAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg > 1:469493/1‑68 (MQ=255) cAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg > 1:278334/1‑68 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:564720/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:3403136/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:2034429/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:2906176/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:2062212/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:689842/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:2696633/43‑1 (MQ=255) gggTTGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:2308448/43‑1 (MQ=255) gggTCGCTGCGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa < 1:3164934/43‑1 (MQ=255) gCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGa < 1:2317326/42‑1 (MQ=255) | GCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCG > minE/2794938‑2795054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |