| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 241,121 | G→A | 37.6% | N1034N (AAC→AAT) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 241,121 | 0 | G | A | 37.6% | 74.8 / 77.5 | 122 | N1034N (AAC→AAT) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (41/35); new base A (30/16); total (71/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
TTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAT > minE/241056‑241183 | ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2720103/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:280635/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2899339/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:2281758/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:717226/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1260393/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1817645/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:3231/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:479375/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:1919259/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:190353/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTg < 1:3228755/69‑1 (MQ=255)ttCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTg > 1:1781231/1‑69 (MQ=255) tCATTATCCTGCTGAATAGTTATTTCATTGCAAACGCACTTTCCAGTATGCTGTAGCCCAGTCCATTg < 1:514840/68‑1 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGGCGTTGAt > 1:1408251/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGa > 1:1629080/1‑68 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:1020227/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:2182684/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:2865706/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:1778231/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:3248476/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:856964/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGAt > 1:455152/1‑69 (MQ=255) cATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCACAGGCCGTTGAt > 1:3376484/1‑69 (MQ=255) aTTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATc > 1:3125601/1‑69 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:3007322/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2567791/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2797596/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:730892/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:512247/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2101935/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:2987739/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:396518/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:3239289/67‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2005819/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:3429108/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:779620/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2895176/68‑1 (MQ=255) tAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGa < 1:2458974/68‑1 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1765779/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1934868/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1858116/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1033669/1‑69 (MQ=255) aTTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCa > 1:1759204/1‑69 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACtt > 1:3314314/1‑57 (MQ=37) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGAt > 1:233559/1‑61 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCt > 1:92309/1‑63 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:3324311/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1550864/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:395627/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1362266/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:3071400/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:52681/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:360481/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2929200/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2281905/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2876781/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:1609019/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2851452/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:2648132/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAcc > 1:89025/1‑68 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCAc > 1:1095361/1‑67 (MQ=255) tCATTGCAAACGCACTTTCCAGTTTGCTGTAGCCAAGTCCATTGATCTTCTTCACTTTGa > 1:2030315/1‑60 (MQ=255) tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCAcc > 1:1640536/1‑68 (MQ=255) aCTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCgg < 1:1158434/68‑1 (MQ=255) aCTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCgg < 1:2771199/68‑1 (MQ=255) tGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTg < 1:1272222/55‑1 (MQ=255) cAAACGTACTTTCCAGTTTGCTGTAGCCCAGGTCGTTGATCtt > 1:3042714/1‑43 (MQ=255) cAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAAt > 1:655197/1‑67 (MQ=255) cAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATAc > 1:2584604/1‑69 (MQ=255) cAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATAc > 1:498491/1‑69 (MQ=255) cAAACGTACTTTCCAGTTTGCTGTAGACCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATAc > 1:574309/1‑69 (MQ=255) gTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGctct < 1:1849505/69‑1 (MQ=255) gTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGctct < 1:2007360/69‑1 (MQ=255) cTTTCCAGTTTGCTGTACCCCAGGCCGTTGATCTTTTTCACttt > 1:1715077/1‑44 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:1533721/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:63468/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:2602322/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:3327722/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:1844986/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:2390377/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:2346315/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:3114638/1‑49 (MQ=255) ttCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGc > 1:306933/1‑49 (MQ=255) tGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTa < 1:2291991/69‑1 (MQ=255) tGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACg < 1:1973234/68‑1 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGcct > 1:2312918/1‑59 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:1009058/1‑62 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:2608184/1‑62 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:2475765/1‑62 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:3213541/1‑62 (MQ=255) tAGCCCAGTCCATTGATCTTCTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:1656509/1‑62 (MQ=255) tAGCCCAGTCCATTGATCTTCCTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCCTc > 1:3086990/1‑62 (MQ=255) gCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCt > 1:45251/1‑69 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:149525/69‑1 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:654519/69‑1 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:477613/69‑1 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:1242142/69‑1 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:105831/69‑1 (MQ=255) cAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAt < 1:1818973/69‑1 (MQ=255) | TTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAAT > minE/241056‑241183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |