| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,796,501 | T→C | 40.0% | N171N (AAT→AAC) | lexA → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,796,501 | 0 | T | C | 40.0% | 54.1 / 89.7 | 125 | N171N (AAT→AAC) | lexA | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (54/21); new base C (15/35); total (69/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
ATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGT > minE/2796433‑2796566 | aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCTAACTGTTGCCAGAAAAt < 1:2938015/69‑1 (MQ=255)aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAAt < 1:3052996/69‑1 (MQ=255)aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAAt < 1:1047698/69‑1 (MQ=255)aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAAt < 1:1867173/69‑1 (MQ=255)aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAAt < 1:3214159/69‑1 (MQ=255)aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAAt < 1:516725/69‑1 (MQ=255) gatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAATTCGAACTGTTGCCAGAAAATa > 1:27724/1‑67 (MQ=255) gaCGAAGTTACCGTTAAACGGCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:682582/69‑1 (MQ=39) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAATGTCGAACTGTTGCCAGAAAACAGCGAg < 1:114752/69‑1 (MQ=39) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:3377522/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:360863/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:3083834/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:1961648/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:1995947/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:2607888/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:70101/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:210564/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:1113655/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:1097187/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:776858/69‑1 (MQ=255) gaCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:2173454/69‑1 (MQ=255) aCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:3302844/68‑1 (MQ=255) aCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:2984268/68‑1 (MQ=255) aCGAAGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAg < 1:1038516/68‑1 (MQ=255) aaGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTa < 1:1617970/69‑1 (MQ=255) aaGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTa < 1:1568791/69‑1 (MQ=255) aaGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTa < 1:368143/69‑1 (MQ=255) aaGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTa < 1:1771601/69‑1 (MQ=255) aaGTTACCGTTAAACGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTa < 1:744946/69‑1 (MQ=255) tACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAAcc > 1:3404312/1‑69 (MQ=255) aCCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCa > 1:1224821/1‑69 (MQ=255) aCCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCa > 1:957935/1‑69 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAg > 1:686828/1‑47 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACATCGAGTTTAAACCAAt > 1:580742/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:3055931/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:2514590/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:472133/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:639525/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:2518680/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:2241041/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:223879/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:1973224/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:1220863/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:1527857/1‑62 (MQ=255) cgcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAACCAAt > 1:1606995/1‑62 (MQ=255) cgcATGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAACAGCGAGTTTAAAc > 1:3121242/1‑58 (MQ=39) gcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg > 1:1361531/1‑69 (MQ=255) gcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg > 1:2451209/1‑69 (MQ=255) gcCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg > 1:2544162/1‑69 (MQ=255) cTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:2208804/69‑1 (MQ=255) cTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:287430/69‑1 (MQ=255) cTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:3048002/69‑1 (MQ=255) cTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:1613607/69‑1 (MQ=255) cTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:3411848/69‑1 (MQ=255) tGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc < 1:2491262/68‑1 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:177785/1‑68 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:1443470/1‑68 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:965314/1‑68 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:2748813/1‑68 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:2375862/1‑68 (MQ=255) aaaaCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc > 1:1027358/1‑68 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGt > 1:1774677/1‑67 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:2055174/1‑68 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:256561/1‑68 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:1624370/1‑68 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:3377327/1‑68 (MQ=255) aCAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:3220648/1‑68 (MQ=255) cAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:2186778/1‑67 (MQ=255) cAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:2057339/1‑67 (MQ=255) cAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:1676426/1‑67 (MQ=255) cAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:2529444/1‑67 (MQ=255) ggCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTcagcag > 1:2907608/1‑69 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:408489/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:546291/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:629781/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:1672543/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:2299085/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:1297381/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:1817480/1‑68 (MQ=255) aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc > 1:920500/1‑68 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:1281910/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:1400906/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:2711056/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:2403659/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:2746172/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:2643979/68‑1 (MQ=255) gTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc < 1:3186303/68‑1 (MQ=255) cTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGaa > 1:1668373/1‑69 (MQ=255) cTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGaa > 1:661051/1‑69 (MQ=255) cTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGaa > 1:867442/1‑69 (MQ=255) gTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg < 1:1289957/69‑1 (MQ=255) gTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg < 1:1060352/69‑1 (MQ=255) gTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg < 1:1050094/69‑1 (MQ=255) gTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg < 1:1469561/69‑1 (MQ=255) gCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCt > 1:1811709/1‑69 (MQ=255) aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc > 1:3424631/1‑38 (MQ=255) aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGt > 1:57099/1‑69 (MQ=255) aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGt > 1:2048713/1‑69 (MQ=255) aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGt > 1:2502208/1‑69 (MQ=255) aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGt > 1:2857166/1‑69 (MQ=255) | ATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGT > minE/2796433‑2796566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |