| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,798,470 | C→A | 35.5% | V136V (GTG→GTT) | zur ← | DNA‑binding transcriptional activator, Zn(II)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,798,470 | 0 | C | A | 35.5% | 56.7 / 40.4 | 59 | V136V (GTG→GTT) | zur | DNA‑binding transcriptional activator, Zn(II)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (19/19); new base A (8/13); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ACTGTTCAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCACGCCT > minE/2798406‑2798533 | aCTGTTCAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATcaca > 1:2306580/1‑68 (MQ=255) gTTCAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTAt > 1:37843/1‑69 (MQ=255) cAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGc > 1:234023/1‑68 (MQ=255) cAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGc > 1:570870/1‑68 (MQ=255) cAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGc > 1:2570909/1‑68 (MQ=255) cAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGc > 1:2648012/1‑68 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:1616699/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:2189260/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:1032378/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:1949700/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:2788095/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:2899810/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:1595505/1‑67 (MQ=255) aGGATGACGACACGCTTCTACTTCTACACATGCTGAACATAATCCATGTGCTTCAATAACATTATGc > 1:533137/1‑67 (MQ=255) gacACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCa < 1:1492052/68‑1 (MQ=255) gacACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCa < 1:1054285/68‑1 (MQ=255) acGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACAtt > 1:2846889/1‑52 (MQ=255) acGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAAc > 1:1912505/1‑68 (MQ=255) acGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAAc > 1:3397452/1‑68 (MQ=255) acGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAAc > 1:1430505/1‑68 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:2437154/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:3128224/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:2427063/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:888447/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:107313/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:1142558/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:1487353/69‑1 (MQ=255) aTGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCg < 1:1818080/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:964161/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:221884/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:735733/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:1102876/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:2593952/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:2670344/69‑1 (MQ=255) tGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGt < 1:2903289/69‑1 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTa < 1:3236023/52‑1 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCAt > 1:2316859/1‑57 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCAt > 1:1611181/1‑57 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:2737187/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:2756029/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:238258/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:1955149/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:2889580/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:3411536/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCAc > 1:1932033/1‑69 (MQ=255) tgCTTCAATCACATTATGCCGCAGGGC‑AACCCCAttttt > 1:2198804/1‑39 (MQ=39) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCTCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2747645/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2951887/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:151979/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2698131/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2472415/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2718682/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:1704740/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:1133402/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2805915/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:178094/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:2352435/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATGATGTCTTCCACGCCt < 1:922796/69‑1 (MQ=255) tCAATAACATTATGCCGCAGGGCAAAACCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCACGCCt < 1:812153/69‑1 (MQ=255) aTCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTa < 1:2242640/45‑1 (MQ=255) | ACTGTTCAGGATGACGACACGCTTCCACTTCTACACATGCCGCACAGAGCCCATGTGCTTCAATCACATTATGCCGCAGGGCAAACCCCATTTTTGCCGCCAGCGTATGCATAATGTCTTCCACGCCT > minE/2798406‑2798533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |