| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,801,019 | C→T | 57.2% | D159D (GAC→GAT) | yjbN → | tRNA‑dihydrouridine synthase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,801,019 | 0 | C | T | 57.2% | 13.9 / 47.7 | 63 | D159D (GAC→GAT) | yjbN | tRNA‑dihydrouridine synthase A |
| Reads supporting (aligned to +/- strand): ref base C (17/10); new base T (14/22); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAG > minE/2800954‑2801085 | tGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCAGCgacgac < 1:717783/69‑1 (MQ=255) cgcgATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGaa > 1:28519/1‑69 (MQ=255) cgcgATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGaa > 1:2998319/1‑69 (MQ=255) gtgtCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGc > 1:2396639/1‑69 (MQ=255) gtgtCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGc > 1:1041136/1‑69 (MQ=255) gtgtCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGc > 1:1066307/1‑69 (MQ=255) gtgtCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGc > 1:3353789/1‑69 (MQ=255) ccGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTtcatc < 1:1981918/69‑1 (MQ=255) ccGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTtcatc < 1:697887/69‑1 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:1590364/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:2343794/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:1731908/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:174787/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:3104722/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:1849667/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:1903123/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:197300/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:2159797/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:2410543/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:468749/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:858890/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:662025/1‑50 (MQ=255) acggtgaAAACGCGTATTGGCATCGATGATCAGGACAGCTATGAATTtct > 1:1031997/1‑50 (MQ=255) aaCGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCg > 1:413749/1‑68 (MQ=255) aaCGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCg > 1:1280408/1‑68 (MQ=255) aaCGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCg > 1:733554/1‑68 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1002546/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:876120/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:2764009/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:2922405/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:799194/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3084209/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1140945/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:423762/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1256892/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3173854/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1286253/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1739827/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3217546/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1708239/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1701023/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:554086/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1451975/69‑2 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:922419/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:2738232/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:748613/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:3097998/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:1363416/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:1217470/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:2104685/69‑1 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCaaatgt < 1:156212/68‑4 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:2306272/68‑2 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1797131/68‑2 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1139508/68‑2 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1137996/68‑2 (MQ=255) ttGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:1065097/68‑1 (MQ=255) aTCGACGAGCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:3408893/1‑67 (MQ=255) aTCGACGACCAGGAGAGCTATGAATTTCTCTGCGATTTCATCAATACAGTTTCCGGCAAAGGCGAgt > 1:695437/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2776741/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2797894/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:1129143/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:1294927/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:188080/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2360573/1‑67 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:824595/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:133577/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:87341/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:1372923/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:3410258/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:168429/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:3308995/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2278925/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2009620/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2187629/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2060544/69‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:2359893/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:894739/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:1091847/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg > 1:2238824/1‑61 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:842812/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:2536293/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:276932/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:1150491/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:2868832/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:49336/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:1760393/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTgagat > 1:1172555/1‑68 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTgag > 1:2046462/1‑69 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2904635/68‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga > 1:1738683/1‑68 (MQ=255) gacgacCAGCACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:3370928/61‑1 (MQ=255) acgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2001828/67‑1 (MQ=255) | TGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAG > minE/2800954‑2801085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |