| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,801,058 | T→C | 30.9% | N172N (AAT→AAC) | yjbN → | tRNA‑dihydrouridine synthase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,801,058 | 0 | T | C | 30.9% | 113.1 / 63.4 | 126 | N172N (AAT→AAC) | yjbN | tRNA‑dihydrouridine synthase A |
| Reads supporting (aligned to +/- strand): ref base T (30/57); new base C (17/22); total (47/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
AACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGG > minE/2801001‑2801124 | aaCGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCg > 1:733554/1‑68 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3217546/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1739827/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:423762/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1286253/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1256892/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:876120/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1708239/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1701023/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1140945/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3173854/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:3084209/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:2922405/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:799194/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:2764009/69‑2 (MQ=255) aTTGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1002546/69‑2 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:1217470/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:748613/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:1363416/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:2104685/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:3097998/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:2738232/69‑1 (MQ=255) aTTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGc < 1:922419/69‑1 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCaaatgt < 1:156212/68‑4 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1797131/68‑2 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1137996/68‑2 (MQ=255) ttGGCATCGATGATCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAggt < 1:1139508/68‑2 (MQ=255) aTCGACGAGCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:3408893/1‑67 (MQ=255) aTCGACGACCAGGAGAGCTATGAATTTCTCTGCGATTTCATCAATACAGTTTCCGGCAAAGGCGAgt > 1:695437/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2797894/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2776741/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:2360573/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:188080/1‑67 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAgt > 1:1294927/1‑67 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:168429/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2278925/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:3308995/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2187629/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2009620/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:3410258/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:824595/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:1372923/69‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:87341/69‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:49336/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:842812/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:894739/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:2536293/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:1091847/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:2359893/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCg < 1:1760393/61‑1 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTgagat > 1:1172555/1‑68 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTgag > 1:2046462/1‑69 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga > 1:1738683/1‑68 (MQ=255) gacgacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTga < 1:2904635/68‑1 (MQ=255) gacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATg < 1:2285798/69‑1 (MQ=255) gacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATg < 1:505303/69‑1 (MQ=255) gacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATg < 1:2016211/69‑1 (MQ=255) gACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTtcatc > 1:1112230/1‑69 (MQ=255) gACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTtcatc > 1:1072018/1‑69 (MQ=255) gACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTtcatc > 1:1068078/1‑69 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:102789/69‑1 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:3104083/69‑1 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:3333704/69‑1 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:289543/69‑1 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:146846/69‑1 (MQ=255) aGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCatcatc < 1:1414521/69‑1 (MQ=255) cTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCca < 1:2281281/69‑1 (MQ=255) aTGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacg < 1:26016/69‑1 (MQ=255) aTGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacg < 1:401480/69‑1 (MQ=255) aTGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacg < 1:855322/69‑1 (MQ=255) tGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgc > 1:360310/1‑69 (MQ=255) tGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgc > 1:2227676/1‑69 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:1986737/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:1987399/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:1470069/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:787228/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:1032384/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCcacgcacg > 1:2704023/1‑68 (MQ=255) tttCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGAGAGTGTGAGATGCTCATCATCcacgcacg > 1:1034067/1‑68 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTga > 1:1572060/1‑46 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATg > 1:583046/1‑50 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:1377911/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:1324350/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:2813025/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:1851472/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:945048/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:1852350/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:1985114/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:3344697/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:2127627/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:3283197/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:2385992/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:3153843/1‑51 (MQ=255) ttCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGTGAGTGTGAGATGt > 1:2790971/1‑51 (MQ=255) aTTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCt < 1:1928970/66‑1 (MQ=255) aTTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCt < 1:3311888/66‑1 (MQ=255) aTTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCt < 1:249210/66‑1 (MQ=255) aaTACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCgggg < 1:1251096/69‑1 (MQ=255) aaTACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCgggg < 1:2456766/69‑1 (MQ=255) tACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCca < 1:2432461/42‑1 (MQ=255) | AACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGG > minE/2801001‑2801124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |