| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,801,121 | G→T | 33.8% | S193S (TCG→TCT) | yjbN → | tRNA‑dihydrouridine synthase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,801,121 | 0 | G | T | 33.8% | 68.7 / 42.9 | 65 | S193S (TCG→TCT) | yjbN | tRNA‑dihydrouridine synthase A |
| Reads supporting (aligned to +/- strand): ref base G (28/15); new base T (11/11); total (39/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
AATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCA > minE/2801056‑2801180 | aaTACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCgggg < 1:2686664/69‑1 (MQ=255)aaTACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCgggg < 1:1251096/69‑1 (MQ=255)aaTACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCgggg < 1:2456766/69‑1 (MQ=255) ccGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTaa > 1:3191300/1‑69 (MQ=255) gCAAAGGTGAGTGTTAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:1629207/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGtcc > 1:2139107/1‑64 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:904947/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:3146562/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:2336126/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:8806/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:1483991/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:2029360/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:1680356/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCgaaa > 1:1598756/1‑68 (MQ=255) gCAAAGGTGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTCCTGGGTTAAGTCCgaaa > 1:1541880/1‑68 (MQ=255) tgAGTGTGAGATTTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:1974096/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGTGTTAAGTCCGAAAGAAAACCg < 1:812852/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:3106924/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:29659/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:1221878/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:3246985/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:3320278/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:789172/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:2159748/68‑1 (MQ=255) tgAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:837290/68‑1 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:1327805/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:3399036/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:1574665/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:199643/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:1646999/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:1818830/1‑66 (MQ=255) tgAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:1814857/1‑66 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTACGCCCGAAAGAAAACCGTGAAATCCcgccgc > 1:503203/1‑68 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGa > 1:3128552/1‑57 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCcgccgc > 1:869426/1‑68 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCcgccgc > 1:1189686/1‑68 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCcgccgc > 1:2072228/1‑68 (MQ=255) catcatCAACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCcgccgc > 1:3330604/1‑68 (MQ=255) catcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCcgccgc < 1:3076096/65‑1 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt > 1:1298103/1‑69 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt > 1:4444/1‑69 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt < 1:894102/69‑1 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt < 1:2716586/69‑1 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt < 1:235353/69‑1 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt > 1:2336674/1‑69 (MQ=255) atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt < 1:345671/69‑1 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaaga > 1:2270166/1‑41 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:2345056/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:124327/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:2506274/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:1452942/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:2473255/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:1529714/1‑64 (MQ=255) cacgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 1:1689932/1‑64 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:1146362/69‑1 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:3178197/69‑1 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:2763306/69‑1 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:1090979/69‑1 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:1594360/69‑1 (MQ=255) gcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcg < 1:667225/69‑1 (MQ=255) cacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcgc > 1:1745841/1‑69 (MQ=255) cacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcgc > 1:301857/1‑69 (MQ=255) cacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcgc > 1:2078953/1‑69 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt < 1:2903919/58‑1 (MQ=255) aGCCTGGCTTTCTGGGTTAAGTCCGAAAGAAAACCg < 1:1454968/36‑1 (MQ=38) gCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTa < 1:1503254/68‑1 (MQ=255) gCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTa < 1:2505829/68‑1 (MQ=255) cTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCa < 1:3168713/69‑1 (MQ=255) | AATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCA > minE/2801056‑2801180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |