| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,801,244 | G→A | 41.2% | E234E (GAG→GAA) | yjbN → | tRNA‑dihydrouridine synthase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,801,244 | 0 | G | A | 41.2% | 40.5 / 68.4 | 97 | E234E (GAG→GAA) | yjbN | tRNA‑dihydrouridine synthase A |
| Reads supporting (aligned to +/- strand): ref base G (33/24); new base A (12/28); total (45/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
CAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTAT > minE/2801179‑2801312 | cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:731375/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:433315/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:1128619/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:263252/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:2319883/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:1896066/1‑68 (MQ=255)cAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc > 1:1845820/1‑68 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2432235/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2856267/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2561131/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2456336/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2357756/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2246121/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:2185675/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:1748677/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:1447024/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:126101/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGc > 1:1410415/1‑67 (MQ=255) aaCTGAAGCGTGACTTTCCGCATCTGACGATGTCGATTAACGGTGGTATCAAGTCGCAGGAAGAAGc > 1:1292528/1‑67 (MQ=255) agtttCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:2032732/67‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGACGAGGCCAAACCACACCTGc < 1:3240817/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCAGACCTGc < 1:3822/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:2800761/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:3031865/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:3093271/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:3182806/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:2897883/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:803688/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:346037/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:26848/69‑1 (MQ=255) aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:1834428/69‑1 (MQ=255) aCTTTCCCCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 1:3106574/69‑1 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAAc > 1:1161896/1‑68 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:3398621/1‑69 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:158404/1‑69 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:2764706/1‑69 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:2408513/1‑69 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:1011511/1‑69 (MQ=255) tCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:822962/1‑69 (MQ=255) aTCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatgg < 1:2094421/69‑1 (MQ=255) tCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatgga > 1:2827747/1‑69 (MQ=255) tCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatgga > 1:93074/1‑69 (MQ=255) tCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatgga > 1:772427/1‑69 (MQ=255) tCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatgga > 1:2694028/1‑69 (MQ=255) cTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatggat < 1:434973/69‑1 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTTGAAGAGGCCAAAGCACACCTGCAACATatggatg > 1:240936/1‑69 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCaa < 1:119444/59‑1 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCaa < 1:2207426/59‑1 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACa > 1:1873651/1‑61 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatggatg > 1:834554/1‑69 (MQ=255) tGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatggatg > 1:2105796/1‑69 (MQ=255) gaTGTCGATTAACTGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1077528/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2927620/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:597985/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:783011/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1072194/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:283690/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:691163/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:388549/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1237930/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:897278/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1347934/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:840794/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1948630/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2140405/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2163416/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2189121/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2667214/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2330269/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2621933/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2640373/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:116106/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2509920/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2504583/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:2492829/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:1188536/67‑1 (MQ=255) gaTGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCAATTGCAACATATGGATGGCg < 1:1886256/67‑1 (MQ=255) gaTGCCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:3112910/67‑1 (MQ=255) tCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGca < 1:3067513/42‑1 (MQ=255) tCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGca < 1:849207/42‑1 (MQ=255) gATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCg < 1:38952/62‑1 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTg > 1:2083622/1‑60 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCggg > 1:1148865/1‑69 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCggg > 1:576831/1‑69 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCggg > 1:671774/1‑69 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCgg > 1:2535652/1‑68 (MQ=255) aaCGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCgg > 1:2847622/1‑68 (MQ=255) aTCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatg < 1:788016/42‑1 (MQ=255) aTCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatg < 1:2533365/42‑1 (MQ=255) aTCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatg < 1:1672006/42‑1 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGGAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGt > 1:787526/1‑67 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGt > 1:338220/1‑67 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGt > 1:2177086/1‑67 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGt > 1:1199609/1‑67 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGt > 1:967010/1‑67 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTa < 1:3195491/68‑1 (MQ=255) aaGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTa < 1:1912438/68‑1 (MQ=255) ggCCAAAGCACACCTGCAACATATCGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTAt > 1:1488714/1‑69 (MQ=255) | CAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTAT > minE/2801179‑2801312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |