| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,801,633 | C→T | 43.4% | intergenic (+53/‑81) | yjbN → / → yjbO | tRNA‑dihydrouridine synthase A/phage shock protein G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,801,633 | 0 | C | T | 43.4% | 19.2 / 50.9 | 69 | intergenic (+53/‑81) | yjbN/yjbO | tRNA‑dihydrouridine synthase A/phage shock protein G |
| Reads supporting (aligned to +/- strand): ref base C (13/26); new base T (13/17); total (26/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAATGCCAGCAAGAGATTTCA > minE/2801571‑2801697 | tAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTg > 1:3356151/1‑69 (MQ=255)tAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTg > 1:3071926/1‑69 (MQ=255)tAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTg > 1:2153423/1‑69 (MQ=255)tAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTg > 1:2149573/1‑69 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGtttt > 1:1965645/1‑55 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTg > 1:2279331/1‑56 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAAt > 1:2449176/1‑65 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:2405131/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:813851/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:652812/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:544533/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:522700/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:40684/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:2832610/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:1894052/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:1051420/1‑66 (MQ=255) ttCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATc > 1:1089970/1‑66 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1792299/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:516220/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1249532/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:3142905/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:3130979/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1333230/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:302260/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:298721/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:2491660/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1417274/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:2315945/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:2200932/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:2013072/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:2072277/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1568680/68‑3 (MQ=255) cACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:1959456/68‑3 (MQ=255) ccAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGTGTTTTGCTGTTAAATCaaca < 1:355088/66‑3 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGatt > 1:199435/1‑69 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGatt > 1:3313363/1‑69 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGat < 1:1723260/68‑1 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGat < 1:3217003/68‑1 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGat > 1:1712508/1‑68 (MQ=255) cAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGat < 1:708604/68‑1 (MQ=255) tagtCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTAttttt > 1:1228282/1‑68 (MQ=255) cAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCAt < 1:2681680/69‑1 (MQ=255) cAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCAt < 1:278396/69‑1 (MQ=255) aaaTTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATg < 1:702311/69‑1 (MQ=255) ttCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGAtt < 1:2115251/69‑1 (MQ=255) ttCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGAtt < 1:132026/69‑1 (MQ=255) ttCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGAtt < 1:2103581/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:3180549/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:706699/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:2208800/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:140368/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:2828380/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:2692440/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:2568924/69‑1 (MQ=255) cacGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:2489062/69‑1 (MQ=255) acGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGa > 1:3250039/1‑46 (MQ=255) acGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTa > 1:1004713/1‑69 (MQ=255) acGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTa > 1:2298423/1‑69 (MQ=255) acGCCCGGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTa > 1:4235/1‑69 (MQ=255) acGCCCGGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTa > 1:434867/1‑69 (MQ=255) gCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTTTAAt < 1:2244046/69‑1 (MQ=255) gCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAAt < 1:1828967/69‑1 (MQ=255) gCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAAt < 1:3035290/69‑1 (MQ=255) gCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAAt < 1:1553482/69‑1 (MQ=255) gCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAAt < 1:1063069/69‑1 (MQ=255) ccGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAATGCCAGCAaga < 1:647706/69‑1 (MQ=255) gggCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATg < 1:1906376/40‑1 (MQ=255) gggCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAATGCCAGCAAGAGATTTCa < 1:546634/68‑1 (MQ=255) ggCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGt < 1:1955883/47‑1 (MQ=255) | TAAGCGTTAACTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATTTTTGGCATGATTCTTGTAATGCCAGCAAGAGATTTCA > minE/2801571‑2801697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |