| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,802,488 | G→A | 62.1% | G206G (GGC→GGT) | qor ← | quinone oxidoreductase, NADPH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,802,488 | 0 | G | A | 62.1% | 57.5 / 71.4 | 108 | G206G (GGC→GGT) | qor | quinone oxidoreductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (10/31); new base A (28/39); total (38/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGC > minE/2802424‑2802545 | aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCTCACTTTCTTACCACCgg < 1:2385649/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTAGCACCgg < 1:4895/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:464929/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1765695/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:20069/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:2674635/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1543658/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:3024892/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:322612/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:3287052/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1266074/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1248090/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:550139/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1129033/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:825810/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:982936/69‑1 (MQ=255)aaTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:1036481/69‑1 (MQ=255) aTCCAGCGACCGTTCCCAGGTGTCTCTGCCCACTGAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:671099/68‑1 (MQ=255) tCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg < 1:546556/69‑1 (MQ=255) tCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg < 1:3209701/69‑1 (MQ=255) tCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg < 1:2781759/69‑1 (MQ=255) tCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg < 1:681434/69‑1 (MQ=255) tCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTg < 1:1597990/69‑1 (MQ=255) cccAGGTGTCTCTGCCCACGGAATCGTACACCACTCGCACTTTCTTACCGCCGGTGATCTCTTTTAAcc > 1:3105414/1‑69 (MQ=255) cccAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAAcc > 1:2201067/1‑69 (MQ=255) cccAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAAcc > 1:991550/1‑69 (MQ=255) gtCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCg > 1:2601474/1‑67 (MQ=255) gtCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCg > 1:1557854/1‑67 (MQ=255) gtCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCg > 1:1412929/1‑67 (MQ=255) gtCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGAc < 1:1126996/69‑1 (MQ=255) tgAATCGTACACCACGCGCACTTTCTTACCACCgg < 1:469747/34‑1 (MQ=38) gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTc > 1:239258/1‑68 (MQ=255) aaTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCAc < 1:95378/69‑1 (MQ=255) aaTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCAc < 1:53877/69‑1 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATctct > 1:2996588/1‑39 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2634201/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:3099105/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1544988/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1539632/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2878201/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2921734/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1748664/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:3118110/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:3127749/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1388461/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:4496/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:635196/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:785114/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:966853/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:982299/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1025979/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:172466/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1873051/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1975728/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2428058/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1597437/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1678277/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2298844/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:2019400/1‑41 (MQ=255) tCGTACACCACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:206065/1‑41 (MQ=255) tCGTACAACACGCGCACTTTCTTACCACCGGTGATCTCttt > 1:1022294/1‑41 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:2134006/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:266072/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:3273131/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:3311516/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:507600/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:544499/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:1237580/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:1190405/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:872571/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:1756027/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:2795372/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:2875734/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:2369571/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:1457770/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:3221047/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTCGCGATAg < 1:2756709/68‑1 (MQ=255) acacCACGCGCACTTTCTTACCACCGGTGATCTCTTTCAATCGCTCGACCAGGTTTTCTTAGCGATAg < 1:3109690/68‑1 (MQ=255) caccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCCCGATAGtt > 1:2560518/1‑69 (MQ=255) caccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGtt < 1:99000/69‑1 (MQ=255) caccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGtt < 1:2239731/69‑1 (MQ=255) caccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGtt < 1:2456125/69‑1 (MQ=255) cacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTtaat < 1:1526302/69‑1 (MQ=255) cacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTtaat < 1:1085871/69‑1 (MQ=255) cacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTtaat < 1:1302123/69‑1 (MQ=255) acGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTtaat < 1:2060906/68‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:1778343/69‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:1349802/69‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:1927403/69‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:936234/69‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:416746/69‑1 (MQ=255) aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGc < 1:888651/69‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:627414/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:1098753/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:2417152/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:3003290/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:1248189/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:2310221/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:136483/41‑1 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATcc < 1:3211672/41‑1 (MQ=255) | AATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGC > minE/2802424‑2802545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |