| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,806,425 | T→C | 37.4% | S146S (AGT→AGC) | tyrB → | tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,806,425 | 0 | T | C | 37.4% | 28.1 / 33.8 | 56 | S146S (AGT→AGC) | tyrB | tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (19/16); new base C (9/12); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01 | |||||||||||
TCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCG > minE/2806358‑2806482 | tCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:894472/69‑1 (MQ=255)tCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:528087/69‑1 (MQ=255)tCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:1553989/69‑1 (MQ=255)tCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:231763/69‑1 (MQ=255)tCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:3001543/69‑1 (MQ=255) cTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTa < 1:427890/68‑1 (MQ=255) gATCCTAGCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg < 1:730984/69‑1 (MQ=255) gATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg < 1:1021908/69‑1 (MQ=255) gATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg < 1:277413/69‑1 (MQ=255) gATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg < 1:2938185/69‑1 (MQ=255) gATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTAATTACCCCTgg < 1:241129/69‑1 (MQ=255) ccTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg > 1:2711459/1‑66 (MQ=255) ccTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg > 1:2776241/1‑66 (MQ=255) aCCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGAc < 1:511721/69‑1 (MQ=255) aCCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGAc < 1:3193182/69‑1 (MQ=255) ttggAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:1738583/3‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTgg < 1:2656393/60‑1 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:2848129/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:3055536/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:1689581/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:331745/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:2413721/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGaa > 1:1175904/1‑69 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGTATGACGa > 1:111321/1‑68 (MQ=255) tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGCACTTACCCCTGGCATGACGaa > 1:1849866/1‑69 (MQ=255) aCGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACg > 1:1599174/1‑69 (MQ=255) aCGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACg > 1:510771/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:2476064/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:3396186/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:3404426/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:1458293/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:3022810/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:1394561/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:674502/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:1204368/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt > 1:2305922/1‑69 (MQ=255) ttCGCCGGGGCTGGATTCGAAGTGAGTACTTACACCTGGTATGACGAAGCGACTAACGGCGTg > 1:1756630/1‑63 (MQ=255) cGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATg < 1:3012622/68‑1 (MQ=255) cGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATg < 1:481305/68‑1 (MQ=255) ttCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCg > 1:315680/1‑69 (MQ=255) tCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGa > 1:630175/1‑58 (MQ=255) tCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGa > 1:2762788/1‑58 (MQ=255) tCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGa > 1:2816265/1‑58 (MQ=255) tCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGa > 1:1883367/1‑58 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:161716/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:3246225/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:1737287/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:3111373/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:1741105/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:2951112/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:55823/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:1861203/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:1391085/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:837712/49‑1 (MQ=255) aGTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:843049/49‑1 (MQ=255) gTGAGCACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCttt < 1:3057369/48‑1 (MQ=255) | TCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCG > minE/2806358‑2806482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |