| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,809,462 | T→C | 42.5% | I117I (ATT→ATC) | yjbQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,809,462 | 0 | T | C | 42.5% | 24.1 / 66.4 | 87 | I117I (ATT→ATC) | yjbQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (21/29); new base C (13/24); total (34/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
GCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTA > minE/2809396‑2809516 | gCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTg < 1:2272980/69‑1 (MQ=255)gCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTg < 1:2965023/69‑1 (MQ=255)gCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTg < 1:2004336/69‑1 (MQ=255) ggAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTg < 1:2701684/65‑1 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:2971754/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:2134211/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:2292517/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:1977246/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:2494225/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:1879671/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:3340382/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:257611/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:3261291/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:3249753/1‑69 (MQ=255) tATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa > 1:2911468/1‑69 (MQ=255) aTTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGc < 1:587521/67‑1 (MQ=255) aTTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGc < 1:3419436/67‑1 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTTGGGGAACATCGCATCCACGGCGGATcg > 1:2195486/1‑68 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCg > 1:3195749/1‑52 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc < 1:1748403/69‑1 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc < 1:3431966/69‑1 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc < 1:2286573/69‑1 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc > 1:2945035/1‑69 (MQ=255) aaGGGCGTATTCAGACCGGCACCTGGCAAGCCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc > 1:1731470/1‑69 (MQ=255) ttaaGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGcatca > 1:1414539/4‑69 (MQ=255) ttCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGa > 1:370622/1‑56 (MQ=255) ttCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGcatca > 1:973314/1‑69 (MQ=255) cAGACCGGGACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1976152/50‑1 (MQ=255) cAGACCGGCACCTGTCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1260142/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2638290/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:3161299/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:3183919/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:3195214/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1335963/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:825765/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:773751/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2745505/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:708830/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1233016/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1667712/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2185894/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:3299407/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2634402/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:331936/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:627282/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:253137/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1917563/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:1953868/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2340795/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:2066332/50‑1 (MQ=255) cAGACCGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCAcgg < 1:457841/50‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGa > 1:682211/1‑50 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:3199682/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:83575/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:2738483/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:463439/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:1855536/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:2995221/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:1289805/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:1781860/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:2742603/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa < 1:2243681/69‑1 (MQ=255) ccGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:2564115/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCg > 1:2040062/1‑58 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:263749/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:48934/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:3152299/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:2377861/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:1812612/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:2599687/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:2770650/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:1486858/1‑69 (MQ=255) ccGGCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:1252390/1‑69 (MQ=255) ccGGCACCTGGAAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:3294755/1‑69 (MQ=255) caggCAGCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa > 1:2166913/3‑69 (MQ=255) caggCACCTGGCAAGGCATCTGGCTGGGGGAACATCGCATCCACGGCGGa > 1:2825379/3‑50 (MQ=255) cGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGac < 1:814736/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:539246/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:2459341/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:67876/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:2761415/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:708373/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:1528380/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:76859/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:3196477/69‑1 (MQ=255) aCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:3077599/69‑1 (MQ=255) ccTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTa < 1:1346503/68‑1 (MQ=255) | GCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTA > minE/2809396‑2809516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |