| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,814,333 | A→G | 31.7% | intergenic (‑418/‑12) | yjcB ← / → yjcC | predicted inner membrane protein/predicted signal transduction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,814,333 | 0 | A | G | 31.7% | 52.1 / 29.6 | 63 | intergenic (‑418/‑12) | yjcB/yjcC | predicted inner membrane protein/predicted signal transduction protein |
| Reads supporting (aligned to +/- strand): ref base A (21/22); new base G (13/7); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTT > minE/2814266‑2814401 | aaGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGaa > 1:659808/1‑69 (MQ=255)aaGATTTATCTAAAGACGTCGGTACCAAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGaa > 1:603210/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCATTGAGTCATc > 1:3080792/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:1021446/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:3369078/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:2281768/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:322369/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:1760979/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:355684/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:3187634/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:3108455/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:1478700/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:488769/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:2594754/1‑69 (MQ=255) cGGTAGCCAGGG‑TTTCACCTTGCAATAACCGGGTATAAACAGGCAGGGAATTGATAGCAATGAGTCATc > 1:656043/1‑69 (MQ=255) aCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTg < 1:3097213/69‑1 (MQ=255) aCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTg < 1:3093138/69‑1 (MQ=255) aCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTg < 1:1976477/69‑1 (MQ=255) aCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTg < 1:1119463/69‑1 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGc > 1:2554444/1‑62 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:2074376/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:3316117/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:1969554/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:2289377/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:2432490/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:464038/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:501819/1‑68 (MQ=255) ttttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca > 1:908047/1‑68 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGATACCAAt < 1:439765/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:3404393/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:2876235/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:3421042/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:458023/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:3002310/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:2911339/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:1272227/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:249436/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:1329331/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:1365494/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:1663858/68‑1 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAt < 1:1890428/68‑1 (MQ=255) aCCTTG‑AATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAAtt < 1:2380075/68‑1 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCa > 1:1013706/1‑67 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCa > 1:3384393/1‑67 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAtt > 1:915771/1‑69 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAtt > 1:76484/1‑69 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAtt > 1:742937/1‑69 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAtt > 1:3234347/1‑69 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAt > 1:2005615/1‑68 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAt > 1:2624632/1‑68 (MQ=255) taAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAt > 1:3434512/1‑68 (MQ=255) aaCAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTa < 1:245130/68‑1 (MQ=255) aaCAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTa < 1:1734054/68‑1 (MQ=255) caggcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCt > 1:2242775/1‑69 (MQ=255) caggcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCt > 1:516627/1‑69 (MQ=255) caggcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCt > 1:1180577/1‑69 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:1727869/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:1334811/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:2132565/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:2515618/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:1237195/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:1150389/69‑1 (MQ=255) ggcaggGAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTTCCGGGCATTATCttt < 1:3032852/69‑1 (MQ=255) gcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCtttt < 1:724717/69‑1 (MQ=255) gcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCtttt < 1:3353566/69‑1 (MQ=255) aaaTTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGtt > 1:2810907/1‑69 (MQ=255) | AAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTT > minE/2814266‑2814401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |