Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,815,784 C→T 49.3% G480G (GGC→GGT yjcC → predicted signal transduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,815,7840CT49.3% ‑0.6 / 66.3 75G480G (GGC→GGTyjcCpredicted signal transduction protein
Reads supporting (aligned to +/- strand):  ref base C (14/24);  new base T (20/17);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTT  >  minE/2815719‑2815852
                                                                 |                                                                    
cATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGt                                                                    >  1:3273973/1‑68 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2663864/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1696906/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1726527/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:666303/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:3143016/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1941330/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:31080/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2040497/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2049035/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1409419/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2857898/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2520623/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:930662/69‑1 (MQ=255)
   ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:2551853/69‑1 (MQ=255)
    tGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1981336/68‑1 (MQ=255)
    tGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:3288983/68‑1 (MQ=255)
        tGCTGAACACATCATCTAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:1037552/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGTTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:2859344/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:1140569/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:1196574/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:2494990/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:3044452/68‑1 (MQ=255)
        tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                            <  1:193281/68‑1 (MQ=255)
               cacaTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAg                                                    <  1:1928155/69‑1 (MQ=255)
                acaTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa                                                                <  1:1620744/56‑1 (MQ=255)
                  atcatcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGtt                                                 <  1:2698648/69‑1 (MQ=255)
                     atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:1727220/1‑69 (MQ=255)
                     atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:2208279/1‑69 (MQ=255)
                     atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:1523601/1‑69 (MQ=255)
                     atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:3335191/1‑69 (MQ=255)
                     atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:550321/1‑69 (MQ=255)
                     atcGACCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc                                              >  1:246671/1‑69 (MQ=255)
                       cGAGCTGGCTCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTg                                            <  1:2168687/69‑1 (MQ=255)
                       cGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTg                                            <  1:2551888/69‑1 (MQ=255)
                                             ttAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc                                                      >  1:1662630/1‑37 (MQ=255)
                                             ttAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc                                                      >  1:2258093/1‑37 (MQ=255)
                                             ttAAAAACGATCGCAGAAGGCGTCGAAACTGAGGAGc                                                      >  1:2867529/1‑37 (MQ=255)
                                             gtAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc                                                      >  1:1734270/2‑37 (MQ=255)
                                               aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa                                  >  1:1846805/1‑55 (MQ=255)
                                               aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa                                  >  1:2199035/1‑55 (MQ=255)
                                               aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa                                  >  1:3347940/1‑55 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTg                                        >  1:722934/1‑40 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:225881/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:2723258/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:2478563/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:2822926/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:2326052/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1285751/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1675548/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:928102/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:677366/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:672050/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:964802/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:627946/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:2344548/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1050603/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1596251/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1691230/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1792080/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1852595/1‑69 (MQ=255)
                                                        cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg           >  1:1948027/1‑69 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:2068033/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:1924131/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:1840583/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:2105733/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:2175592/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:1640985/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:230089/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:1456224/65‑1 (MQ=255)
                                                        cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc               <  1:1421126/65‑1 (MQ=255)
                                                           tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt         <  1:188424/68‑1 (MQ=255)
                                                           tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt         <  1:1503947/68‑1 (MQ=255)
                                                           tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt         <  1:1192364/68‑1 (MQ=255)
                                                           tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt         <  1:264305/68‑1 (MQ=255)
                                                                 cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt  <  1:3035819/69‑1 (MQ=255)
                                                                 cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt  <  1:1788538/69‑1 (MQ=255)
                                                                 cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt  <  1:906366/69‑1 (MQ=255)
                                                                 |                                                                    
CATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTT  >  minE/2815719‑2815852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: