| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,817,164 | T→C | 20.7% | A95A (GCT→GCC) | nrfA → | nitrite reductase, formate‑dependent, cytochrome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,817,164 | 0 | T | C | 20.7% | 128.3 / 24.5 | 91 | A95A (GCT→GCC) | nrfA | nitrite reductase, formate‑dependent, cytochrome |
| Reads supporting (aligned to +/- strand): ref base T (45/27); new base C (5/14); total (50/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.42e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACGGCTGGTGATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATG > minE/2817096‑2817225 | ccACGGCTGGTGATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCt > 1:3098426/1‑69 (MQ=255)ccACGGCTGGTGATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCt > 1:1610387/1‑69 (MQ=255) tGATCCTGTGGGCAGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCCTTTGCtgtg > 1:1181342/1‑68 (MQ=255) tGATCCTGTGGGCAGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCCTTTGCTGTGa > 1:1047984/1‑69 (MQ=255) tGATCCTGTGGGCAGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCCTTTGCTGTGa > 1:2031712/1‑69 (MQ=255) tGATCCTGTGGGCAGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCCTTTGCTGTGa > 1:298522/1‑69 (MQ=255) tGATCCTGTGGGCAGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCCTTTGCTGTGa > 1:1503669/1‑69 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:2654239/1‑68 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:939794/1‑68 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:2954516/1‑68 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:191563/1‑68 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:423950/1‑68 (MQ=255) gATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:17145/1‑68 (MQ=255) gATCCTGTGGGCGGAGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGa > 1:585867/1‑68 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:940331/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:2729603/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:2785136/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:2814507/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:2122044/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:1104785/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:1260360/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:1609463/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGc > 1:720145/1‑60 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:1108795/1‑69 (MQ=255) ccTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:70089/1‑69 (MQ=255) ggCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGAtgtg < 1:2594198/67‑1 (MQ=255) ggCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGAtgtg < 1:2960120/67‑1 (MQ=255) cGGTGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:1852959/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCTCTTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:776532/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:3435940/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:2595165/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:2716346/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:22899/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:2076481/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:947027/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:1192915/69‑1 (MQ=255) cGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:1144187/69‑1 (MQ=255) cGGGCTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:2940203/69‑1 (MQ=255) gTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAAc > 1:2887545/1‑69 (MQ=255) gTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAAc > 1:1392214/1‑69 (MQ=255) gTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAAc > 1:1959433/1‑69 (MQ=255) gTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAAc > 1:37528/1‑69 (MQ=255) cccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTg > 1:2814583/1‑69 (MQ=255) cccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTg > 1:2750730/1‑69 (MQ=255) cccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTg > 1:254032/1‑69 (MQ=255) cccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTg > 1:237234/1‑69 (MQ=255) cccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTg > 1:2289441/1‑69 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCtgt < 1:2661843/46‑1 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCtgt < 1:76980/46‑1 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:1817767/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:3015859/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:3407624/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:2155815/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:1464517/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:2244585/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:942423/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:120767/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:2530678/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:2467706/1‑52 (MQ=255) ccTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGa > 1:2494895/1‑52 (MQ=255) ctcGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:324735/56‑1 (MQ=255) gCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTg < 1:1689283/38‑1 (MQ=255) cgcgTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:461454/69‑1 (MQ=255) cgcgTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:997420/69‑1 (MQ=255) cgcgTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:2766531/69‑1 (MQ=255) cgcgTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:1875975/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:572190/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:1314768/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:890392/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:13042/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:75940/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:734653/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:234316/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:703081/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:971057/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:2069187/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:3377591/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:3162075/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:1779190/69‑1 (MQ=255) cgcgTGGACATGCCTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTg < 1:1980208/69‑1 (MQ=255) tGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:2242730/69‑1 (MQ=255) tGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:2426842/69‑1 (MQ=255) tGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:1700132/69‑1 (MQ=255) tGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:1983869/69‑1 (MQ=255) ggACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:667670/68‑1 (MQ=255) ggACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGa < 1:976150/68‑1 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:2436588/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:1319453/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:142812/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:2799865/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:1478096/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:2816026/1‑69 (MQ=255) gACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATg > 1:1733889/1‑69 (MQ=255) | CCACGGCTGGTGATCCTGTGGGCGGGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGATGTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATG > minE/2817096‑2817225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |