| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,824,650 | A→G | 37.5% | Y107Y (TAT→TAC) | yjdC ← | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,824,650 | 0 | A | G | 37.5% | 8.2 / 11.4 | 24 | Y107Y (TAT→TAC) | yjdC | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (4/5); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAATCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATAAATAGACAGCCCGGATAGCGGTTGTTTTTAACGCACTCCGAT > minE/2824586‑2824713 | tGAAATCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATaa < 1:3235615/68‑1 (MQ=255)tGAAATCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATaa < 1:2862634/68‑1 (MQ=255) aTCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACg < 1:1220428/68‑1 (MQ=255) aTCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACg < 1:3430676/68‑1 (MQ=255) aTCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACg < 1:323730/68‑1 (MQ=255) aTCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACg < 1:2726351/68‑1 (MQ=255) gTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTAc > 1:554145/1‑68 (MQ=255) ttttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGGTAAAACGTACAGGCAGCGATa > 1:1058724/1‑68 (MQ=255) ttttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGGTAAAACGTACAGGCAGCGATa > 1:958995/1‑68 (MQ=255) ttttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGGTAAAACGTACAGGCAGCGATa > 1:101911/1‑68 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGGTAAAACGTACAGGCAGCGATa > 1:1991208/1‑67 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATa > 1:2288615/1‑67 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATa > 1:2241157/1‑67 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATa > 1:1847578/1‑67 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATa > 1:525908/1‑67 (MQ=255) tttttGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATa > 1:715317/1‑67 (MQ=255) ttGATGAATAGGGTGGCCAGGATCGGGATAAAACg < 1:687682/35‑1 (MQ=255) ggCCATGATCGGGGTAAAACGTACAGGCAGCGATAAACAGACAGCCCGGATAg < 1:485893/53‑1 (MQ=255) ggCCAGGATCGGGGTAAAACGTACAGGCAGCGATAAACAGACAGCCCGGATAg < 1:2574662/53‑1 (MQ=255) ggCCAGGATCGGGGTAAAACGTACAGGCAGCGATAAACAGACAGCCCGGATAg < 1:312814/53‑1 (MQ=255) ggCCAGGATCGGGGTAAAACGTACAGGCAGCGATAAACAGACAGCCCGGATAg < 1:118020/53‑1 (MQ=255) gCCAGGATCGGGGTAAAACGTACAGGCAGCGATAAACAGACAGCCCGGATAg < 1:552399/52‑1 (MQ=255) gCCAGGATCGGGATAAAACGTACAGGCAGCGATAAATAGACAGCCCGGATAGCGGTTGTTTTTAACGCa < 1:756419/69‑1 (MQ=255) tCGGGATAAAACGTACAGGCAGCGATAAATAGACAGCCCGGATAGCGGTTGTTTTTAACGCACTCCGAt < 1:3070091/69‑1 (MQ=255) | TGAAATCGTAGGCCGCGCTTTTTTGCTGATCGGCCAGTTGATGAATAGGGTGGCCAGGATCGGGATAAAACGTACAGGCAGCGATAAATAGACAGCCCGGATAGCGGTTGTTTTTAACGCACTCCGAT > minE/2824586‑2824713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |