| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,827,128 | T→G | 48.0% | intergenic (‑110/+6) | cutA ← / ← dcuA | copper binding protein, copper sensitivity/C4‑dicarboxylate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,827,128 | 0 | T | G | 48.0% | ‑4.1 / 59.8 | 50 | intergenic (‑110/+6) | cutA/dcuA | copper binding protein, copper sensitivity/C4‑dicarboxylate antiporter |
| Reads supporting (aligned to +/- strand): ref base T (8/18); new base G (22/2); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01 | |||||||||||
ACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACC > minE/2827060‑2827187 | aCTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt > 1:1152129/1‑69 (MQ=255)aCTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt > 1:1180575/1‑69 (MQ=255) tataCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTg < 1:2973109/68‑1 (MQ=255) tataCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTg < 1:201300/68‑1 (MQ=255) cggccggGGGAAGGATGTTAGTCGGCGGGAAAGAACGCGGGGCGTGAACGCCCCGCGATTg < 1:3235957/61‑1 (MQ=255) gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAg < 1:1824026/69‑1 (MQ=255) gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAg < 1:1466991/69‑1 (MQ=255) acggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAg < 1:3405454/68‑1 (MQ=255) acggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAg < 1:552107/68‑1 (MQ=255) gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTa > 1:2430903/1‑68 (MQ=255) gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTa > 1:2216165/1‑68 (MQ=255) gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTa > 1:2545313/1‑68 (MQ=255) gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTa > 1:2894791/1‑68 (MQ=255) gTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTg < 1:2696403/41‑1 (MQ=255) gaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCa < 1:366607/69‑1 (MQ=255) gaaagCGGGGCGTGAACGCCCCGCGATGGGTCATTACAGCATGAAGCTAccc < 1:3431399/52‑1 (MQ=255) gggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCa < 1:2410737/62‑1 (MQ=255) gggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCa < 1:2316294/62‑1 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa < 1:1757448/69‑1 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa > 1:271253/1‑69 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa > 1:2735703/1‑69 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa > 1:1455565/1‑69 (MQ=255) aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa > 1:731070/1‑69 (MQ=255) aCGCCCCGCGATGGGTCATTACAGCATGAAGCTAccc < 1:639918/37‑1 (MQ=255) ccccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:562780/69‑1 (MQ=255) ccccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:2615339/69‑1 (MQ=255) ccccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:1463415/69‑1 (MQ=255) ccccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:133271/69‑1 (MQ=255) ccccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:1125829/69‑1 (MQ=255) cccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc < 1:871833/68‑1 (MQ=255) ccGCGATGGGTCATTACAGGATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2573130/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGCAGCTACCCAGc > 1:1653500/1‑35 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAATCAAACGGCCAGGGCAACAcc > 1:1054498/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:906410/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:718179/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:402438/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:80865/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:339044/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:3343571/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:3223850/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:3044705/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2869715/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2834582/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:276293/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2328466/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2307778/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:2303997/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:1444504/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:1127669/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:1095756/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:1063012/1‑67 (MQ=255) ccGCGATGGGTCATTACAGCATGAAGCCACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc > 1:1857297/1‑67 (MQ=255) | ACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACC > minE/2827060‑2827187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |