BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,835,378	G→T	27.1%	D173E (GAC→GAA)	yjeJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,835,378	0	G	T	27.1%	161.2 / 52.2	103	D173E (GAC→GAA)	yjeJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (46/29); new base T (18/10); total (64/39)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 103 total aligned reads displayed.

ATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTA  >  minE/2835312‑2835433

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:943987/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:239975/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:2403442/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:2102636/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:2602370/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:811650/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:695182/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:3185181/69‑1 (MQ=255)

aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc                                                       <  1:326800/69‑1 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTgg                                                   >  1:2414724/1‑68 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:2282596/1‑69 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:2176215/1‑69 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:1479428/1‑69 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:514136/1‑69 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:926802/1‑69 (MQ=255)

     ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc                                                  >  1:1203576/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:14785/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:217313/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:885669/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:2461169/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:2832063/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:2022104/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:2018241/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:1972166/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:63110/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:1783532/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:3327888/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:1704479/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:1580593/1‑69 (MQ=255)

      gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa                                                 >  1:554845/1‑69 (MQ=255)

            cAAGTTATTTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc                                           >  1:848613/1‑69 (MQ=255)

            cAAGTTATTTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc                                           >  1:852563/1‑69 (MQ=255)

            cAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc                                           >  1:684434/1‑69 (MQ=255)

            cAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc                                           >  1:912912/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:2705246/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:401348/69‑1 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:471271/69‑1 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:593122/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:1054045/69‑1 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:1595553/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:125501/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:21953/69‑1 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:1221436/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:1220870/69‑1 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     >  1:2151330/1‑69 (MQ=255)

                                  tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt                     <  1:2071258/69‑1 (MQ=255)

                                     gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCTGTCAACGTCGTATAACGGCAGGAAATCTaa                   <  1:1018928/68‑1 (MQ=255)

                                     gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTaa                   <  1:2426318/68‑1 (MQ=255)

                                     gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTaa                   <  1:3036144/68‑1 (MQ=255)

                                     gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAAGTCGTATAACGGCAGGAAATCTaa                   <  1:3250382/68‑1 (MQ=255)

                                      aaTAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACa                 >  1:3316982/1‑69 (MQ=255)

                                        tAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGt               <  1:1261732/69‑1 (MQ=255)

                                         aGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTg              <  1:1371073/69‑1 (MQ=255)

                                         aGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGAAGGAAATCTAACAGTg              <  1:681283/69‑1 (MQ=255)

                                          ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGt               <  1:2708764/67‑1 (MQ=255)

                                          ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa             >  1:413612/1‑69 (MQ=255)

                                          ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa             >  1:251211/1‑69 (MQ=255)

                                          ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa             >  1:778683/1‑69 (MQ=255)

                                          ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa             >  1:1217554/1‑69 (MQ=255)

                                            tgtCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAg            >  1:2520159/1‑68 (MQ=255)

                                              tCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGt          >  1:808358/1‑68 (MQ=255)

                                              tCATATTCCAGATTGATATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGt          >  1:2146162/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:1210889/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:1539953/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:1463365/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:675693/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:3092080/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:2291767/1‑68 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg    <  1:804933/69‑1 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg    <  1:2663060/69‑1 (MQ=255)

                                                   ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg    <  1:2548610/69‑1 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc     >  1:1124398/1‑66 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:1430872/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:557455/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:1460789/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:1964835/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:3158710/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2064221/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2766911/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2061479/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2847701/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2065506/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:2087081/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:311098/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:1986801/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:1759287/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:3413816/1‑69 (MQ=255)

                                                     ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa  >  1:355577/1‑69 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGTCAGGAAATCTAACAGTGAGGTAATACGt   <  1:2307435/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:2269104/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:855583/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:2269441/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:376217/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:2662324/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:758316/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:303153/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:143420/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:224680/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt   <  1:554729/68‑1 (MQ=255)

                                                     ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAAAACGt   <  1:3008921/68‑1 (MQ=255)

ATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTA  >  minE/2835312‑2835433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`