| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,839,277 | G→A | 44.1% | A36A (GCC→GCT) | blc ← | outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,839,277 | 0 | G | A | 44.1% | 8.0 / 30.3 | 43 | A36A (GCC→GCT) | blc | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (8/16); new base A (10/9); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACG > minE/2839223‑2839337 | ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:414954/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:1401321/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:1579742/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:2478162/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:1943089/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:2097289/69‑1 (MQ=255)ttCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAattatt < 1:2043164/69‑1 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:2445334/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:741569/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:542831/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:37614/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:2778911/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:2508401/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:1052550/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:237047/1‑58 (MQ=255) cAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:2005706/1‑58 (MQ=255) cAAAGCGGTCATAAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTc > 1:3055376/1‑58 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:1084999/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:1109339/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:481825/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:46855/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:1264958/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:2922032/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:2687725/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:1694153/68‑1 (MQ=255) aaGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTAGCGTCGAAATTATTcac < 1:2200567/68‑1 (MQ=255) gTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGt < 1:1457933/69‑1 (MQ=255) gTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGt < 1:2911278/69‑1 (MQ=255) gTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGt < 1:2229621/69‑1 (MQ=255) cAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCg > 1:3202222/1‑47 (MQ=255) cAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTcactc < 1:2617586/69‑3 (MQ=255) cAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTcacgc > 1:2551526/1‑69 (MQ=255) cAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTcacgc > 1:2586510/1‑69 (MQ=255) cAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTcacgc > 1:2178459/1‑69 (MQ=255) ctcATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGt < 1:2532340/69‑1 (MQ=255) tcATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGt > 1:1431083/1‑68 (MQ=255) aCCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGc > 1:2007241/1‑68 (MQ=255) aCCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCa > 1:660216/1‑69 (MQ=255) aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAg < 1:2001754/50‑1 (MQ=255) aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAg < 1:3318107/50‑1 (MQ=255) aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAg < 1:2489938/50‑1 (MQ=255) aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAg < 1:1572475/50‑1 (MQ=255) aCGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACg > 1:1870449/1‑67 (MQ=255) | TTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTTTGGCGTCGAAATTATTTACTACGGTCACGCCACGCGGCGGCGTAGGAGAACTGCAGGCAACG > minE/2839223‑2839337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |