| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,839,488 | A→G | 41.4% | L373L (CTT→CTC) | ampC ← | beta‑lactamase/D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,839,488 | 0 | A | G | 41.4% | 10.6 / 27.2 | 41 | L373L (CTT→CTC) | ampC | beta‑lactamase/D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base A (11/13); new base G (6/11); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCA > minE/2839421‑2839546 | gACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTa > 1:2216741/1‑68 (MQ=255) gAAAAGCGGAGAAAAGGTCCGAATATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGc > 1:3427789/1‑68 (MQ=255) gAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGcc > 1:972076/1‑69 (MQ=255) gAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGcc > 1:2935723/1‑69 (MQ=255) gAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGcc > 1:2622779/1‑69 (MQ=255) gAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGcc > 1:3381693/1‑69 (MQ=255) ctaaaaTTCGGACCCGATGGAATTTTTCTGTAGAGCGTTAAGAATCTGCCAGGCGGCGtg < 1:2031654/58‑2 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:358097/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:1616377/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:2072472/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:1412847/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:626521/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:3190809/1‑58 (MQ=255) aaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:196801/69‑1 (MQ=255) aaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:3360471/69‑1 (MQ=255) aaTTCGTACCCGATGGATTTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:2424583/68‑1 (MQ=255) aaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc < 1:468141/56‑1 (MQ=255) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:406329/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:342263/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2941116/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2819132/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:1260785/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2642217/1‑48 (MQ=38) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:450178/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:2705753/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3175508/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3206381/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:2473209/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:1681018/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3424435/69‑1 (MQ=255) tttACAGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:1311422/69‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTTGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2136937/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1975702/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1814121/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2195627/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2814618/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1570881/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1364654/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:765093/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:840717/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:871082/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2905296/66‑1 (MQ=255) | GACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCA > minE/2839421‑2839546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |