| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,839,507 | T→G | 38.7% | D367A (GAC→GCC) | ampC ← | beta‑lactamase/D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,839,507 | 0 | T | G | 38.7% | 15.0 / 37.3 | 44 | D367A (GAC→GCC) | ampC | beta‑lactamase/D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base T (15/12); new base G (6/11); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 | |||||||||||
CGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATA > minE/2839449‑2839575 | ctaaaaTTCGGACCCGATGGAATTTTTCTGTAGAGCGTTAAGAATCTGCCAGGCGGCGtg < 1:2031654/58‑2 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:3190809/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:358097/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:1412847/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:1616377/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:626521/1‑58 (MQ=255) aaaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc > 1:2072472/1‑58 (MQ=255) aaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:3360471/69‑1 (MQ=255) aaaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:196801/69‑1 (MQ=255) aaTTCGTACCCGATGGATTTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTgg < 1:2424583/68‑1 (MQ=255) aaTTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTc < 1:468141/56‑1 (MQ=255) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2642217/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:406329/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:342263/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2819132/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:2941116/1‑48 (MQ=38) aaTTTTACTGTAGAGCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCg > 1:1260785/1‑48 (MQ=38) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3175508/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:2705753/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3206381/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:3424435/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:2473209/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:450178/69‑1 (MQ=255) tttACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:1681018/69‑1 (MQ=255) tttACAGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTtttgtttg < 1:1311422/69‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTTGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2136937/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:765093/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:840717/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:871082/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2905296/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2814618/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1364654/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1570881/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1814121/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:2195627/66‑1 (MQ=255) gagCGTTGAGAATCTGCCAGGCGGCGGCGACTCTCGCTGGATTGGGGTAGTTTTTGTTAGCCAGCa < 1:1975702/66‑1 (MQ=255) aTCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGttt > 1:1225736/1‑42 (MQ=255) aTCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGc > 1:605736/1‑69 (MQ=255) aTCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGc > 1:393036/1‑69 (MQ=255) aTCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGc > 1:77637/1‑69 (MQ=255) aTCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGc > 1:827891/1‑69 (MQ=255) tCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGct > 1:2123671/1‑69 (MQ=255) tCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGct > 1:656656/1‑69 (MQ=255) tCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGct > 1:750675/1‑69 (MQ=255) tCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGct > 1:330767/1‑69 (MQ=255) tCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATa < 1:741345/69‑1 (MQ=255) tCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATa < 1:2307558/69‑1 (MQ=255) tCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATa < 1:2616897/69‑1 (MQ=255) | CGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAATCTGCCAGGCGGCGTCGACTCTCGCTGGATTGGGATAGTTTTTGTTTGCCAGCATCACGATACCCAGCTCTTTTTCTGGAATA > minE/2839449‑2839575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |