| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,841,988 | A→G | 70.0% | G64G (GGT→GGC) | frdB ← | fumarate reductase (anaerobic), Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,841,988 | 0 | A | G | 70.0% | 82.3 / 35.7 | 84 | G64G (GGT→GGC) | frdB | fumarate reductase (anaerobic), Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/6); new base G (22/36); total (41/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCT > minE/2841930‑2842045 | cACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATc > 1:23984/1‑68 (MQ=255)cACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATc > 1:28537/1‑68 (MQ=255) aCGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGc < 1:875413/69‑1 (MQ=255) aGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGc > 1:563039/1‑69 (MQ=255) tGCCAGTTTTGGCACGTT‑TTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:2892156/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:2020885/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:2577514/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:2545048/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:274951/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:281115/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3152857/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3220300/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3263271/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3345050/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3364026/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3414257/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:503442/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:827379/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:163400/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:1438489/69‑1 (MQ=255) gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:1344187/69‑1 (MQ=255) ccAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3046731/68‑1 (MQ=255) ttGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAgg > 1:3240921/1‑54 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCGGGACCAGCGGTAGCTCAGGTCCGGt > 1:732098/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGAc > 1:8559/1‑47 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:3084120/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2809497/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2348648/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1530288/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1481245/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:67687/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2860916/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2668304/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2736609/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:144809/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1433237/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2116325/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:467696/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:2003235/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:853828/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1955073/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1946420/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1670806/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1637802/1‑68 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:3012820/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:335037/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:44418/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2864890/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:1217143/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:76836/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2663467/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2603998/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2571342/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2468115/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2465178/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2266517/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2258659/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:208700/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:2235651/69‑1 (MQ=255) tgttAACCATCATGCCGCAGGACCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:444276/69‑1 (MQ=255) tgctAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGt > 1:1174270/4‑68 (MQ=255) aaCCATCATGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg < 1:50741/65‑1 (MQ=255) aaCCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg < 1:2761213/69‑1 (MQ=255) aaCCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg < 1:1104797/69‑1 (MQ=255) aaCCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg < 1:63291/69‑1 (MQ=255) aaCCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg < 1:115400/69‑1 (MQ=255) aaCCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg < 1:2800739/69‑1 (MQ=255) atcatGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:1968220/45‑1 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGTTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1411295/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1096931/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:3393967/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1262078/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:3318702/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1373864/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1453434/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1479457/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1043021/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:2142472/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1536165/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1980709/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:188460/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCt > 1:1853898/1‑69 (MQ=255) atGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGAGGTAGCTCAGGACCGGTGCCAGGTTGTCt > 1:1978637/1‑69 (MQ=255) tGCCGCAGGAGCCACAAATCGCCATACGGCAGGACCAGCgg < 1:3047083/41‑1 (MQ=255) | CACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCT > minE/2841930‑2842045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |