| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 244,761 | C→T | 30.1% | G221R (GGG→AGG) | sbcD ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 244,761 | 0 | C | T | 30.1% | 28.7 / 11.1 | 33 | G221R (GGG→AGG) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (15/8); new base T (5/5); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244704‑244829 | aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGt < 1:1023213/69‑1 (MQ=255)aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGt < 1:563957/69‑1 (MQ=255)aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGt < 1:432448/69‑1 (MQ=255)aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGt < 1:3307036/69‑1 (MQ=255) gCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGt < 1:622280/68‑1 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCTGTGAATATGCCTGAGCGCGATGTAGTCGGCtg > 1:2304818/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCtg > 1:732096/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCtg > 1:640391/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCtg > 1:1592722/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCtg > 1:3159517/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtg > 1:103191/1‑69 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtg > 1:971778/1‑69 (MQ=255) ccgccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa > 1:2249199/1‑44 (MQ=255) ccgccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGc > 1:2215185/1‑68 (MQ=255) ccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa < 1:665098/69‑1 (MQ=255) ccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa < 1:2024687/69‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa > 1:861083/1‑37 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa < 1:1382442/67‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:78190/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:1529616/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:1670325/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:432982/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:170145/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa < 1:396146/67‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa < 1:2867321/67‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa < 1:2848167/67‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa < 1:2772155/67‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGaaa > 1:2464318/1‑67 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcg > 1:2064730/1‑69 (MQ=255) atCTGTGCTCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcgcg > 1:2784263/1‑69 (MQ=255) atCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcgcg > 1:2660694/1‑69 (MQ=255) tgtgCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcgc > 1:2961432/1‑65 (MQ=255) tGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:3382874/68‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt < 1:3243822/69‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt < 1:168916/69‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt < 1:1530828/69‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt < 1:796505/69‑1 (MQ=255) | AGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244704‑244829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |