Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,845,871	G→A	28.8%	R124H (CGT→CAT)	yjeM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,845,871	0	G	A	28.8%	79.3 / 31.1	76	R124H (CGT→CAT)	yjeM	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (39/15);  new base A (5/17);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 1.03e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTC  >  minE/2845806‑2845935                                                                  |                                                                 cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:2179564/1‑69 (MQ=255) cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:2978694/1‑69 (MQ=255) cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:2977106/1‑69 (MQ=255) cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:753063/1‑69 (MQ=255) cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:197774/1‑69 (MQ=255) cTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:1337565/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:841482/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:3224203/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:3056100/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:945400/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:1156990/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:959716/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAtt                                                              >  1:611088/1‑69 (MQ=255)  tCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTAt                                                               >  1:3200898/1‑68 (MQ=255)   ccGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTg                                                             >  1:3188613/1‑69 (MQ=255)   ccGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTg                                                             >  1:2366409/1‑69 (MQ=255)   ccGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTg                                                             >  1:1092812/1‑69 (MQ=255)   ccGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTg                                                             >  1:2662136/1‑69 (MQ=255)   ccGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTg                                                             >  1:2676380/1‑69 (MQ=255)                  tACCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACT‑GCATATTGCCGGACTGGAGCCTAc                                             <  1:1585976/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:2932393/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:274391/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:26926/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:31009/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:2731393/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:2094125/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:499300/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:1967745/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:1527320/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:755544/69‑1 (MQ=255)                   aCCGTTCTCAACATTCCTCTACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:763808/69‑1 (MQ=255)                      gTTCTCAACATTCCTCTACGGTAGCGACATGTCCCAGCACTGGCATATTGCCGGACTGGAGCCTAc                                             <  1:719417/66‑1 (MQ=255)                              cATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:2985877/69‑1 (MQ=255)                              cATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:1379444/69‑1 (MQ=255)                               aTTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttgg                                 <  1:1188770/69‑1 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:539756/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:2818107/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:1134005/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:1171275/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:1244319/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:145979/1‑69 (MQ=255)                                ttCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttggt                                >  1:2090173/1‑69 (MQ=255)                                  ccTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:115046/65‑1 (MQ=255)                                  ccTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:2686242/65‑1 (MQ=255)                                  ccTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:472175/65‑1 (MQ=255)                                     ctACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTctgct                           <  1:603570/69‑1 (MQ=255)                                     ctACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTctgct                           <  1:2994119/69‑1 (MQ=255)                                     ctACGGTAGCGACATGACCCAGCACTGGCATATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTctgct                           <  1:3134408/69‑1 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctgtctg                      >  1:2838906/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:2056379/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:617078/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:1718223/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:2009169/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:267290/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggctg                      >  1:2499367/1‑69 (MQ=255)                                          gTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTgctggct                       >  1:1671229/1‑68 (MQ=255)                                              cGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttg                                  <  1:3131433/53‑1 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:238923/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:27996/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:2361449/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:2860598/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:2631817/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:455245/1‑69 (MQ=255)                                                  aTGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATgg              >  1:3364502/1‑69 (MQ=255)                                                     aCCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGtggttt                                  <  1:2303347/46‑2 (MQ=255)                                                     aCCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGgatg           >  1:1351570/1‑69 (MQ=255)                                                     aCCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGgatg           >  1:2694358/1‑69 (MQ=255)                                                         aGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTc       >  1:2433503/1‑69 (MQ=255)                                                          gCACTGGCATATTGCCGGACTGGAGCCTACGCAGGTGTTTGGTctgct                           <  1:1991046/48‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:1990532/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:3007754/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:731335/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:163247/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:2802759/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:865434/69‑1 (MQ=255)                                                              tGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTc  <  1:2698779/69‑1 (MQ=255)                                                                  |                                                                 CTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGTATTGCCGGACTGGAGCCTACGCAGGTGGTTGGTCTGCTGGCTGTGGCATGGATGATTCTGGTC  >  minE/2845806‑2845935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑