BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,846,388	T→C	25.5%	N296N (AAT→AAC)	yjeM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,846,388	0	T	C	25.5%	146.3 / 48.3	125	N296N (AAT→AAC)	yjeM	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (54/39); new base C (15/17); total (69/56)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ATGGTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGA  >  minE/2846321‑2846451

aTGGTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATg                                                                <  1:3248081/69‑1 (MQ=255)

  ggTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCa                                                              >  1:356916/1‑69 (MQ=255)

  ggTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCa                                                              >  1:845216/1‑69 (MQ=255)

  ggTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCa                                                              >  1:3050434/1‑69 (MQ=255)

   gTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGc                                                               >  1:2640497/1‑67 (MQ=255)

   gTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCAc                                                             >  1:2677381/1‑69 (MQ=255)

   gTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCAc                                                             >  1:2771972/1‑69 (MQ=255)

     tCCGTTAACCTCGG‑AATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:3320684/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:2314517/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:1785602/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:357561/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:1870381/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:1317271/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:18773/69‑1 (MQ=255)

      ccGTTAACCTCGGCAATATTACCTATGTGCCGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGc                                                          <  1:3105162/69‑1 (MQ=255)

        gTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCa                                                         <  1:1481501/68‑1 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:321249/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1889231/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:353721/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1996410/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1070999/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:2932754/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:2361654/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:2598841/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:187963/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:883816/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1154141/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1385702/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAtt                                                       >  1:1306938/1‑69 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAt                                                        >  1:662375/1‑68 (MQ=255)

         ttAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTCGGGGTGACGCTGGGTAACGCACTGCAt                                                        >  1:837047/1‑68 (MQ=255)

              cTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCa                                                  >  1:1438322/1‑69 (MQ=255)

              cTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCa                                                  >  1:1214186/1‑69 (MQ=255)

                       atTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATg                                                                >  1:419799/1‑46 (MQ=255)

                        ttACCTATGTTCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:1688411/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCg                                         >  1:1861775/1‑68 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2593243/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:932181/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2657532/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2700061/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2819060/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2870510/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:814993/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:527614/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:478030/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:42519/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:36808/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:1987676/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:1272820/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:1898586/1‑69 (MQ=255)

                        ttACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGt                                        >  1:2010730/1‑69 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCTTTTGTCACCTGAAGCGTc                                       <  1:570782/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:2306380/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:1981623/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:1989943/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:1551488/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:566843/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:3174851/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:3071603/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:685780/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:721393/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:138606/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:2385783/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:135990/68‑1 (MQ=255)

                          aCCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:248667/68‑1 (MQ=255)

                                 tgCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGt                                                    >  1:1949024/1‑48 (MQ=255)

                                 tgCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGt                                                    >  1:3111145/1‑48 (MQ=255)

                                 tgCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTc                                                   >  1:2487128/1‑49 (MQ=255)

                                 tgCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTc                                                   >  1:2763022/1‑49 (MQ=255)

                                 tgCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTc                                                   >  1:1634471/1‑49 (MQ=255)

                                     gatgaAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTggg                           >  1:1061250/1‑69 (MQ=255)

                                     gatgaAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTggg                           >  1:2643129/1‑69 (MQ=255)

                                                     aTGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGcgcg            <  1:2563648/68‑1 (MQ=255)

                                                     aTGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGcgcg            <  1:612694/68‑1 (MQ=255)

                                                     aTGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGcgcg            <  1:3003894/68‑1 (MQ=255)

                                                        aCGCTGGGTAATGCAGTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:3036786/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:1393481/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:25608/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:3219922/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:2458667/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:2345404/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:3196426/1‑69 (MQ=255)

                                                        aCGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        >  1:2951195/1‑69 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCTTAtt        <  1:3081724/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:2540888/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:3291514/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:2496054/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:843308/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:782032/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:753684/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:2322933/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:2267261/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:1550697/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:440808/69‑1 (MQ=255)

                                                        aCGCTGGGTAACGCACTGCATTTTTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:1724705/69‑1 (MQ=255)

                                                         cGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTAtt        <  1:648335/68‑1 (MQ=255)

                                                          gCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTc                                       <  1:219854/36‑1 (MQ=255)

                                                             gggTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTgg   <  1:3109693/69‑1 (MQ=255)

                                                             gggTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTgg   <  1:1086251/69‑1 (MQ=255)

                                                                tAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGa  <  1:3278280/67‑1 (MQ=255)

ATGGTTCCGTTAACCTCGGCAATATTACCTATGTGCTGATGAAGAGCCTTGGGATGACGCTGGGTAATGCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGA  >  minE/2846321‑2846451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`