Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,846,814 A→G 55.5% T438T (ACA→ACG)  yjeM → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,846,8140AG55.5% 34.8 / 102.3 128T438T (ACA→ACG) yjeMpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base G (9/62);  total (37/91)
Fisher's exact test for biased strand distribution p-value = 9.86e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-02

Only 100 of 128 total aligned reads displayed.

AGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGT  >  minE/2846756‑2846873
                                                          |                                                           
atgATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:2123342/65‑1 (MQ=255)
aGGATCTCGACAGACCGTTTTTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1283056/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCATAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1424347/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:755015/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:723124/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:814165/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1677708/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:2316519/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1949165/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1435599/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:909982/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1953156/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:1339429/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:2024942/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:2028779/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:2085855/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:49994/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:212257/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGt                                                     <  1:97339/67‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:3191049/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:611305/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:185189/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:2372787/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:3169391/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:734615/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:376175/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:118202/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:82971/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:958668/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:2564144/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:770618/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:3025560/69‑1 (MQ=255)
aGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGGCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:1320996/69‑1 (MQ=255)
 ggATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:1591449/68‑1 (MQ=255)
  gATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGt                                                  <  1:1721030/68‑1 (MQ=255)
  gATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGt                                                  <  1:501699/68‑1 (MQ=255)
    tctcGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCg                                                   <  1:1832380/65‑1 (MQ=255)
            gACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGa                                       <  1:1927807/69‑1 (MQ=255)
            gACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGa                                       <  1:2924129/69‑1 (MQ=255)
            gACCGTTTGTGATTTTCAAAACGCATTTCTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGa                                       <  1:2990955/69‑1 (MQ=255)
                   tgtgATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTg                                 >  1:1002934/1‑68 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTTGTGGTGACATTTGCGAAc                            <  1:897515/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:2562129/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:2471215/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:2576960/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:3042616/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:2827913/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:3407285/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:2010322/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1884434/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1840654/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1810607/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1620955/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1467958/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1325543/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:934921/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1149582/69‑1 (MQ=255)
                       aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAAc                            <  1:1068714/69‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:3400117/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:69684/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:817024/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:919850/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:2311877/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:1419231/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:2509998/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:2434169/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:2275487/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:1817861/68‑1 (MQ=255)
                            cAAAACGCATTTGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCt                        <  1:2117037/68‑1 (MQ=255)
                                 cGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCAcc                   >  1:1488253/1‑68 (MQ=255)
                                 cGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCAcc                   >  1:167659/1‑68 (MQ=255)
                                 cGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCAcc                   >  1:1721661/1‑68 (MQ=255)
                                 cGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCAcc                   >  1:2903468/1‑68 (MQ=255)
                                 cGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCAcc                   >  1:1882102/1‑68 (MQ=255)
                                  gCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcat                 <  1:1004760/69‑1 (MQ=255)
                                  gCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcat                 <  1:2544953/69‑1 (MQ=255)
                                    aTTTGTCGGCAATGATTGCGACGGTGGTTGTCGTTCTGGTGGTGACATTTGCGAACGTCt                        <  1:1769018/60‑1 (MQ=255)
                                     tttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcatca               >  1:1694240/1‑68 (MQ=255)
                                     tttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcatca               >  1:782832/1‑68 (MQ=255)
                                     tttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcatca               >  1:2187889/1‑68 (MQ=255)
                                     tttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcatca               >  1:692461/1‑68 (MQ=255)
                                     tttGTCGGCAATCATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACcatca               >  1:963526/1‑68 (MQ=255)
                                       tGTCGGCAATGTTTGCGACGGTGGTTGTCGTACtggt                                            >  1:522170/1‑37 (MQ=38)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:1510912/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:2431511/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:1771577/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:1404637/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:551785/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:217403/1‑69 (MQ=255)
                                       tGTCGGCAATGATTGCGACGGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTc            >  1:2131211/1‑69 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTGTTCACCATCATTCAACCTGTGGt  >  1:717475/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTgg   >  1:1294612/1‑67 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTgg   >  1:3097612/1‑67 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:1280288/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:1874397/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:178130/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:345843/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:3220986/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGt  >  1:2727796/1‑68 (MQ=255)
                                                  aTTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACAATCATTCAACCTGTGGt  >  1:441772/1‑68 (MQ=255)
                                                          |                                                           
AGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGT  >  minE/2846756‑2846873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑