| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,848,531 | C→T | 45.3% | V834V (GTG→GTA) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,848,531 | 0 | C | T | 45.3% | 16.5 / 69.3 | 86 | V834V (GTG→GTA) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base C (11/36); new base T (27/12); total (38/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACA > minE/2848466‑2848595 | tCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac > 1:3394587/1‑68 (MQ=255)tCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac > 1:3093044/1‑68 (MQ=255) gcgggcggGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2540/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGa > 1:2521609/1‑67 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2605357/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:147849/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1563571/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1580682/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:160038/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:164836/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2049023/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2085673/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2432070/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1017290/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2815643/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2905661/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:2913996/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:3086624/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:3367930/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:899463/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:916327/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:133252/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1242471/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1043184/1‑68 (MQ=255) gcgGGCAGGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAg > 1:1461870/1‑68 (MQ=255) ggCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTa < 1:1288726/69‑1 (MQ=255) ggCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTa < 1:1049679/69‑1 (MQ=255) tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:3409575/1‑68 (MQ=255) tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1002674/1‑68 (MQ=255) tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1186792/1‑68 (MQ=255) tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:360464/1‑68 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2755/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:1798601/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:651918/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:807179/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3209838/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2509663/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3196451/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2492933/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:105877/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:2614914/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:109800/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:567886/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:647648/69‑1 (MQ=255) aGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:1411105/68‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAgtgt < 1:1156966/49‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:3201034/49‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:952259/49‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:3380309/49‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:1602552/49‑1 (MQ=255) gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt < 1:2573449/49‑1 (MQ=255) gatgatAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTg > 1:196470/1‑69 (MQ=255) atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:789629/1‑69 (MQ=255) atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:3224917/1‑69 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:812738/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:448923/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2145824/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2250571/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:225535/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2839088/69‑1 (MQ=255) gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa < 1:3175436/69‑1 (MQ=255) tGGCTATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:483355/43‑1 (MQ=25) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1939040/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2584699/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1609684/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1347142/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:62344/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:50455/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:391969/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3355190/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2287889/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3086932/43‑1 (MQ=37) cAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1271763/40‑1 (MQ=37) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1335482/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1878512/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1404143/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1400146/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:3222526/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1160019/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1290977/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:2641474/1‑68 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTTACa < 1:1840017/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:1527349/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:897231/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2338328/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:117581/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2640119/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACACCCTGTACCGTGGAGGTGACa < 1:2784814/60‑1 (MQ=255) | TCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACA > minE/2848466‑2848595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |