| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,848,549 | T→C | 24.9% | P828P (CCA→CCG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,848,549 | 0 | T | C | 24.9% | 74.7 / 20.7 | 64 | P828P (CCA→CCG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base T (20/28); new base C (4/12); total (24/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGA > minE/2848485‑2848613 | tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:360464/1‑68 (MQ=255)tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1186792/1‑68 (MQ=255)tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:3409575/1‑68 (MQ=255)tGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGTACCGCACCGAGGGTAATCGGc > 1:1002674/1‑68 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2492933/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3196451/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:807179/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2509663/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:3209838/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:651918/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:1798601/69‑1 (MQ=255) aCCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa < 1:2755/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:105877/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:2614914/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:567886/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:647648/69‑1 (MQ=255) cAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:109800/69‑1 (MQ=255) aGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACt < 1:1411105/68‑1 (MQ=255) gatgatAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTg > 1:196470/1‑69 (MQ=255) atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:3224917/1‑69 (MQ=255) atAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc > 1:789629/1‑69 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2839088/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2145824/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:2250571/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:812738/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:448923/69‑1 (MQ=255) aaaCACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt < 1:225535/69‑1 (MQ=255) gAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa < 1:3175436/69‑1 (MQ=255) tGGCTATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:483355/43‑1 (MQ=25) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1609684/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3086932/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1347142/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:62344/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:3355190/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:50455/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2584699/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1939040/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:391969/43‑1 (MQ=37) tGGCAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:2287889/43‑1 (MQ=37) cAATCAGTACCGCACCGAGGGTAATCGGCTCCAGGCTTTc < 1:1271763/40‑1 (MQ=37) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:3222526/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:2641474/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1290977/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1335482/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1400146/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1160019/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1404143/1‑68 (MQ=255) cAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCcaca > 1:1878512/1‑68 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTTACa < 1:1840017/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:117581/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:1527349/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2338328/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:2640119/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACa < 1:897231/60‑1 (MQ=255) aGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACACCCTGTACCGTGGAGGTGACa < 1:2784814/60‑1 (MQ=255) gcaccgAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAata > 1:854512/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGATGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:2943028/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:2993764/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:965277/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:1214973/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:61820/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:2414044/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:2905367/1‑69 (MQ=255) tAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa > 1:2968095/1‑69 (MQ=255) | TGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGA > minE/2848485‑2848613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |