| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,849,359 | A→G | 100% | G558G (GGT→GGC) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,849,359 | 0 | A | G | 82.3% | 60.4 / 3.8 | 39 | G558G (GGT→GGC) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base G (3/29); total (10/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCA > minE/2849302‑2849423 | gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1198793/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:616970/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:456787/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:2878719/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:2786483/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:2323530/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:2267117/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:2247199/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1943234/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1924667/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1667180/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1136807/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1249949/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:1253110/68‑1 (MQ=255)gcgagcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCaa < 1:131827/68‑1 (MQ=255) ggCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAg > 1:862621/1‑69 (MQ=255) ggCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAg > 1:589646/1‑69 (MQ=255) ggCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAg > 1:122163/1‑69 (MQ=255) ggCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAg > 1:2690707/1‑69 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGTTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:2664341/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1292682/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:230688/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1971130/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:2603551/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:2607618/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1428427/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:2748091/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1901401/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:3381999/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGTGCCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1471452/69‑1 (MQ=255) gcAAATCATCACTACCCACAGCAGCGGCACGGCGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:3113664/69‑1 (MQ=255) cAAATCATCACTACCCACAGCAGCGGCACGGTGGCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:1673885/68‑1 (MQ=255) cacaGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTcg > 1:1582855/1‑69 (MQ=255) gcagcGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCa > 1:1821245/1‑69 (MQ=255) gcagcGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCa > 1:999650/1‑69 (MQ=255) ggCTGTTACGCCATCACCAATCGCGACCGCCAGCgg < 1:581266/36‑1 (MQ=25) ttACGCCATCACCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCa > 1:292872/1‑69 (MQ=255) ttACGCCATCACCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCa > 1:1218615/1‑69 (MQ=255) ttACGCCATCACCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCa > 1:2893010/1‑69 (MQ=255) | GCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCA > minE/2849302‑2849423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |