| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,849,437 | T→C | 61.2% | L532L (TTA→TTG) ‡ | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,849,437 | 0 | T | C | 61.2% | 47.7 / 57.8 | 90 | L532L (TTA→TTG) ‡ | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base T (12/23); new base C (28/27); total (40/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGA > minE/2849380‑2849494 | cAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGAcgc < 1:3294596/69‑1 (MQ=255) gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCg > 1:3010554/1‑69 (MQ=255) gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCg > 1:394533/1‑69 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2941652/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2878756/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2789904/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:1209160/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:526849/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2005999/69‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:758859/69‑1 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:1010508/69‑1 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2830446/69‑1 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2155012/69‑1 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:835744/69‑1 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGAcg > 1:2375218/1‑48 (MQ=37) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGcgac > 1:3251745/1‑52 (MQ=37) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGcgac > 1:2247250/1‑52 (MQ=37) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGaa > 1:191439/1‑57 (MQ=38) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3206656/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2623081/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:102835/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3228621/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:675827/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:935083/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3304974/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3099172/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:900757/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2927585/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1021139/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:577664/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1047415/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:696125/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1815414/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1631528/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1607266/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:726438/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:193267/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1956539/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1974755/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2069313/1‑67 (MQ=255) cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGa > 1:3307516/1‑66 (MQ=255) cgcgCAAGCCGTAAACCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1924270/1‑67 (MQ=255) ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1950881/69‑1 (MQ=255) ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:261578/69‑1 (MQ=255) ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:2065956/69‑1 (MQ=255) ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1372000/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCCGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:717044/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGAGGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:857916/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:963446/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:948170/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:310667/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:315634/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:3223272/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:893727/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:568812/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:8757/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2941483/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1885324/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1945041/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2173155/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:153473/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2332320/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1332738/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1280785/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2422952/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2517510/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2545329/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2580048/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1085541/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2783877/69‑1 (MQ=255) cccAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2534561/68‑1 (MQ=255) aGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:283303/65‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:2589377/69‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:1105471/69‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:3018518/69‑1 (MQ=255) ccACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1879777/57‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGGCAGAAGTGa < 1:2263383/69‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg > 1:2635357/1‑68 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1644688/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1855052/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa < 1:1011114/69‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:796199/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1911952/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:642764/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2253769/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2362629/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:3392612/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:318481/1‑69 (MQ=255) caGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAag < 1:2930818/42‑1 (MQ=255) aCCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2766777/37‑1 (MQ=255) | CAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGA > minE/2849380‑2849494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |