| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,849,476 | C→T | 71.5% | L519L (TTG→TTA) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,849,476 | 0 | C | T | 71.5% | 60.8 / 25.8 | 63 | L519L (TTG→TTA) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base C (10/8); new base T (17/28); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTC > minE/2849408‑2849532 | ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:2065956/69‑1 (MQ=255)ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1950881/69‑1 (MQ=255)ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1372000/69‑1 (MQ=255)ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:261578/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCCGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:717044/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGAGGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:857916/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:310667/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2173155/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2332320/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2422952/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2517510/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2580048/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2783877/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2941483/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2545329/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:315634/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:3223272/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:568812/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:8757/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:893727/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:948170/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:963446/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1085541/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:153473/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1332738/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1885324/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1945041/69‑1 (MQ=255) aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1280785/69‑1 (MQ=255) cccAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2534561/68‑1 (MQ=255) aGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:283303/65‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:1105471/69‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:3018518/69‑1 (MQ=255) ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:2589377/69‑1 (MQ=255) ccACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1879777/57‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGGCAGAAGTGa < 1:2263383/69‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg > 1:2635357/1‑68 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2253769/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:318481/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1644688/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:3392612/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1855052/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:642764/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa < 1:1011114/69‑1 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1911952/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:796199/1‑69 (MQ=255) cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2362629/1‑69 (MQ=255) agcGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2184816/42‑1 (MQ=39) gAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTc < 1:2297923/69‑1 (MQ=255) gAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTc < 1:745557/69‑1 (MQ=255) aaaaGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTc < 1:1460667/68‑1 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCa > 1:555701/1‑39 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:2620749/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:106271/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:1077950/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:2058762/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:883612/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:2428714/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:745908/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:1554256/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:2671802/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:613322/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:518818/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:2852725/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:3337707/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAGCCAGAAGTGATCCTGAGTCAc > 1:3242770/1‑40 (MQ=255) aaaGAGTGCGTAACGTCAACCAGAAGTGATCCTGAGTCAc > 1:1999490/1‑40 (MQ=255) aaaGAGAGCGTAACGTCAGCCAGAAGTGATCCTGAGt > 1:1837411/1‑37 (MQ=38) | CCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTC > minE/2849408‑2849532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |