| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,851,132 | A→T | 41.0% | P297P (CCT→CCA) | psd ← | phosphatidylserine decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,851,132 | 0 | A | T | 41.0% | 15.1 / 35.1 | 39 | P297P (CCT→CCA) | psd | phosphatidylserine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base A (10/13); new base T (12/4); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGA > minE/2851065‑2851200 | cTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAg > 1:2865381/1‑69 (MQ=255)cTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAg > 1:2130001/1‑69 (MQ=255)cTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAg > 1:1815059/1‑69 (MQ=255) aaCCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTgg > 1:899995/1‑69 (MQ=255) aaCCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTgg > 1:2859055/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTgg > 1:3195833/1‑43 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:2638473/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:949384/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:1115106/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:2403429/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:1982515/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:1971906/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:1502550/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:1745254/1‑69 (MQ=255) cgtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGAGTCTGGCGTAACAAAGGttt > 1:2413100/1‑69 (MQ=255) cgtcgtGTTCTGCATCGATCTCTTAAGCAGGAAGCGGGGCTGGTTCACCGt > 1:2842701/1‑51 (MQ=255) cgtcatGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGttt > 1:2071098/1‑69 (MQ=255) gtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGTTTc < 1:1887504/69‑1 (MQ=255) gtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGTTTc < 1:3319605/69‑1 (MQ=255) gtcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGTTTc < 1:1338503/69‑1 (MQ=255) tcgtGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCTGGTTCAGCGTCTGGCGTAACAAAGGTTTc < 1:1354697/68‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:899116/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:1557154/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:431816/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:2463265/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:1500186/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:2913691/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:1994772/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:3200427/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:3227130/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:3274953/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:3307037/69‑1 (MQ=255) ttCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGa < 1:3348945/69‑1 (MQ=255) cTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTGTGGCGTAACAAAGGTTTCGGTAGATa < 1:3198191/69‑1 (MQ=255) ctctTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCgg > 1:299969/1‑69 (MQ=255) ctctTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCgg > 1:1807714/1‑69 (MQ=255) aGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTc > 1:2656441/1‑69 (MQ=255) aGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTc > 1:560393/1‑69 (MQ=255) aGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTAGGTAGATACTGCCAGCGGCTGGCCAATTTTc > 1:2586893/1‑69 (MQ=255) aGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTgacaga < 1:676925/69‑1 (MQ=255) aGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTgacaga < 1:1313218/69‑1 (MQ=255) | CTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGATCTCTTCAGCAGGAAGCGGGGCAGGTTCAGCGTCTGGCGTAACAAAGGTTTCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGA > minE/2851065‑2851200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |