| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,854,647 | C→T | 38.4% | L361L (TTG→TTA) | yjeS ← | predicted Fe‑S electron transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,854,647 | 0 | C | T | 38.4% | 17.8 / 22.2 | 39 | L361L (TTG→TTA) | yjeS | predicted Fe‑S electron transport protein |
| Reads supporting (aligned to +/- strand): ref base C (6/18); new base T (10/5); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTC > minE/2854579‑2854715 | cTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGc < 1:2661660/69‑1 (MQ=255)cTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGc < 1:2862048/69‑1 (MQ=255)cTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGc < 1:442473/69‑1 (MQ=255)cTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGc < 1:3097952/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGTAATTg < 1:67357/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGTAATTg < 1:549688/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGTAATTg < 1:1523988/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGTAATTg < 1:1801820/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTg < 1:2852244/69‑1 (MQ=255) gATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTg < 1:138280/69‑1 (MQ=255) aTGAATCATGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGTAATTg < 1:2575860/68‑1 (MQ=255) gAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCAc > 1:2108823/1‑69 (MQ=255) ggCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGAc > 1:468019/1‑69 (MQ=255) ggCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGAc > 1:763814/1‑69 (MQ=255) aaCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCAttt > 1:3325733/1‑69 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1158844/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2552945/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:794428/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:123378/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1603856/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2116875/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1865837/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2418921/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1891284/68‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:835764/67‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1127586/67‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1586240/67‑1 (MQ=255) tCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTAttt > 1:777116/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGAcc > 1:382908/1‑41 (MQ=39) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:296791/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:131832/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2766498/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2653249/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2648339/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2642364/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2258613/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2568991/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2472206/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGAc < 1:485177/41‑1 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGAc < 1:2585789/41‑1 (MQ=255) aaCCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2068060/59‑1 (MQ=255) cAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAAt < 1:1848239/69‑1 (MQ=255) gCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGcc > 1:239483/1‑68 (MQ=255) cAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTc < 1:1523506/69‑1 (MQ=255) | CTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTC > minE/2854579‑2854715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |