| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,854,664 | T→C | 63.6% | I356V (ATA→GTA) ‡ | yjeS ← | predicted Fe‑S electron transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,854,664 | 0 | T | C | 63.6% | 39.7 / 35.6 | 63 | I356V (ATA→GTA) ‡ | yjeS | predicted Fe‑S electron transport protein |
| Reads supporting (aligned to +/- strand): ref base T (5/18); new base C (9/31); total (14/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
AACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCT > minE/2854606‑2854732 | aaCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCAttt > 1:3325733/1‑69 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2116875/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1891284/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:794428/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1158844/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:123378/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2418921/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1865837/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2552945/68‑1 (MQ=255) aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1603856/68‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1586240/67‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:835764/67‑1 (MQ=255) aTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:1127586/67‑1 (MQ=255) tCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTAttt > 1:777116/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2568991/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2653249/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2648339/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2642364/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2766498/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:296791/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2472206/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:2258613/1‑69 (MQ=255) aCCCGAACCAGACGCTGTTTTTTCGGTAATTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTtgc > 1:131832/1‑69 (MQ=255) aaCCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTa < 1:2068060/59‑1 (MQ=255) cAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAAt < 1:1848239/69‑1 (MQ=255) gCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGcc > 1:239483/1‑68 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:760022/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2801920/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2808574/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1429939/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:3150039/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:3172502/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:3333228/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:3420124/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2832643/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:777811/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1145575/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:866135/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:978742/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:994408/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:998628/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2516528/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2095978/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:194372/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2133725/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1919569/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2399941/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1874225/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1873320/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1074309/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2598956/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1639766/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1612705/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:1579033/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2751448/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2751734/66‑1 (MQ=255) gtaaTTGCACTTCGACCACGCACGCATTTCGCCTCTCGATTTGCTGCGCAATCGCCCACGCTATGTGc < 1:2755457/66‑1 (MQ=255) cAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTc < 1:1523506/69‑1 (MQ=255) tGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGc > 1:779006/1‑56 (MQ=255) tGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGc > 1:1139063/1‑56 (MQ=255) cTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga < 1:773294/68‑1 (MQ=255) cTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga < 1:2974026/68‑1 (MQ=255) tATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTg < 1:846820/69‑1 (MQ=255) tGCACGCATTTCTTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCt > 1:941051/1‑69 (MQ=255) tGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGc > 1:1402848/1‑68 (MQ=255) tGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCt > 1:629692/1‑69 (MQ=255) | AACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCT > minE/2854606‑2854732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |